We narrowed to 72,194 results for: LAS

1. Intron-Tagging-pX330-Cas9-Blast

   Plasmid

   #159741

   Purpose

   Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti PGK GFP Blast (w510-5)

   Plasmid

   #19069

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Blast

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNA motif plasmid cloning backbone

   Plasmid

   #107253

   Purpose

   RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

   Depositor

   Paul Khavari

   Article

   Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

   Insert

   RNA motif plasmid cloning backbone
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIP-UbC-Blast-2A-STING-mNeonGreen

   Plasmid

   #227185

   Purpose

   Lentiviral expression plasmid encoding STING-mNeonGreen under UbC promoter

   Depositor

   Nir Hacohen

   Article

   Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

   Insert

   STING1 (STING1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF-FH-TAZ S89A-ires-blast

   Plasmid

   #52084

   Purpose

   Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

   Depositor

   Yutaka Hata

   Article

   Yang et al Mol Cell Biol. 2014 Feb 18.

   Insert

   TAZ S89A (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   S89A

   Promoter

   EF1a

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7 promoter + filler DNA reporter plasmid

   Plasmid

   #156454

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGMC00025 (aka pCW-dCas9-Blast)

   Plasmid

   #195316

   Purpose

   Dox inducible lentiviral dead Cas9

   Depositor

   Raj Chari

   Insert

   dead Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   D10A; H840A

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PGK Blast DEST (w524-1)

   Plasmid

   #19065

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRM166 LKB (Luciferase mKate2 Blast)

   Plasmid

   #183502

   Purpose

   Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   mKate2
   BSD
   

   Use

   Lentiviral and Luciferase

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiTet-OTX2-P2A-mCherry BlastR

    Plasmid

    #186741

    Purpose

    Dox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    OTX2 (OTX2 Human)
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-mCherry-WT-KRAS-IRES-Blast

    Plasmid

    #153335

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    mCherry-WT-KRAS (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMVTRE3G eGFP Blast (w818-1)

    Plasmid

    #27568

    Depositor

    Eric Campeau

    Insert

    Enhance Green Fluorescent Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV 4xSTAR-sTomato-NLS-blast

    Plasmid

    #136259

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2T-CMV-evoCjCas9-TadCDd-BlastR

    Plasmid

    #202558

    Purpose

    Mammalian expression of evoCjCas9 TadCDd

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-TadCDd
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV 4xSTAR-mNeonGreen-NLS-blast

    Plasmid

    #136258

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV 4xSTAR-mScarletI-NLS-blast

    Plasmid

    #136260

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mScarletI-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-NLS-copGFP-EF1-BlastiS

    Plasmid

    #132772

    Purpose

    Spills into the cytoplasm during nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    NLS-copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-eGFP-SV40p-BlasR

    Plasmid

    #228271

    Purpose

    pLenti-CMV-eGFP-SV40p-BlasR plasmid for ectopic expression of GFP

    Depositor

    Judy Lieberman

    Article

    Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLY094-EFS-CD22-Blast-WPRE

    Plasmid

    #192195

    Purpose

    Lenti-CD22-OE-Blast

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-CD22-OE-Blast (CD22 Synthetic)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CMV-eA3A-BE5-NG-BlastR

    Plasmid

    #152998

    Purpose

    High purity C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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