We narrowed to 36,563 results for: Mut

1. pCBLsgRNA1

   Plasmid

   #199727

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU3-sgRNA1 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBLsgRNA2

   Plasmid

   #199728

   Purpose

   Golden Gate entry vector; 2nd gRNA under OsU3 promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   OsU3-sgRNA2 scaffold
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. prNFL-EGFP

   Plasmid

   #132605

   Purpose

   Expresses EGFP-tagged rat NFL

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. prNFL-EGFP

   Plasmid

   #132605

   Purpose

   Expresses EGFP-tagged rat NFL

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEYFP-N1-HsSmg5_H

   Plasmid

   #146474

   Purpose

   Mammalian Expression of HsSmg5

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg5 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   three non silent mutation A254G (K85R), T980C (L3…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEYFP-N1-HsSmg5_H

   Plasmid

   #146474

   Purpose

   Mammalian Expression of HsSmg5

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg5 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   three non silent mutation A254G (K85R), T980C (L3…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p23-DDX21 SAT

   Plasmid

   #128804

   Purpose

   overexpression of DDX21 SAT mutation in human cells

   Depositor

   Ling-Ling Chen

   Article

   Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

   Insert

   DDX21 SAT mutation
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p23-DDX21 SAT

   Plasmid

   #128804

   Purpose

   overexpression of DDX21 SAT mutation in human cells

   Depositor

   Ling-Ling Chen

   Article

   Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

   Insert

   DDX21 SAT mutation
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMB66

   Plasmid

   #47946

   Purpose

   Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMB66

    Plasmid

    #47946

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE30-TEV-pro-conA

    Plasmid

    #159527

    Purpose

    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

    Depositor

    Joshua Mylne

    Article

    Nonis et al bioRxiv. 2020.

    Insert

    Canavalia ensiformis pro-concanavalin A
    

    Use

    Tags

    Six-Histidine tag and tobacco etch virus (TEV) pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE30-TEV-pro-conA

    Plasmid

    #159527

    Purpose

    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.

    Depositor

    Joshua Mylne

    Article

    Nonis et al bioRxiv. 2020.

    Insert

    Canavalia ensiformis pro-concanavalin A
    

    Use

    Tags

    Six-Histidine tag and tobacco etch virus (TEV) pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Venus-Bik-DD-pEGFP-C1

    Plasmid

    #177429

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Threonine 33 and Serine 35 replaced with aspartic…

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus-Bik-DD-pEGFP-C1

    Plasmid

    #177429

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Threonine 33 and Serine 35 replaced with aspartic…

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPL5628_TEF1*-Cre_MET15

    Plasmid

    #60933

    Purpose

    Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPL5628_TEF1*-Cre_MET15

    Plasmid

    #60933

    Purpose

    Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p23-DDX21 DEV

    Plasmid

    #128805

    Purpose

    overexpression of DDX21 DEV mutation in human cells

    Depositor

    Ling-Ling Chen

    Article

    Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

    Insert

    DDX21 DEV mutation
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p23-DDX21 DEV

    Plasmid

    #128805

    Purpose

    overexpression of DDX21 DEV mutation in human cells

    Depositor

    Ling-Ling Chen

    Article

    Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

    Insert

    DDX21 DEV mutation
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge