We narrowed to 91,244 results for: cloning

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pLGB28

Plasmid

#126617

Purpose

pNBU2-derived shuttle vector for integration at attBT2 sites in Bacteroides chromosomes, inulin selection for use in anbitiotic resistant strains

Depositor

Laurie Comstock

Publication

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Unspecified; Chromosomal integration suicide vect…

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLGB28

Plasmid

#126617

Purpose

pNBU2-derived shuttle vector for integration at attBT2 sites in Bacteroides chromosomes, inulin selection for use in anbitiotic resistant strains

Depositor

Laurie Comstock

Publication

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Unspecified; Chromosomal integration suicide vect…

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCVL-UCOE-SFFV-TLR-MCV1

Plasmid

#117111

Purpose

Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.

Depositor

Erik Sontheimer

Publication

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

UCOE SFFV Traffic Light Reporter 2.0

Use

Lentiviral; Reporter

Expression

Mammalian

Promoter

SFFV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCVL-UCOE-SFFV-TLR-MCV1

Plasmid

#117111

Purpose

Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.

Depositor

Erik Sontheimer

Publication

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

UCOE SFFV Traffic Light Reporter 2.0

Use

Lentiviral; Reporter

Expression

Mammalian

Promoter

SFFV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pN-PITCh-GFP

Plasmid

#127888

Purpose

Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

Depositor

Peter Kaiser

Publication

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

Puro-GFP

Use

CRISPR

Expression

Mammalian

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pN-PITCh-GFP

Plasmid

#127888

Purpose

Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

Depositor

Peter Kaiser

Publication

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

Puro-GFP

Use

CRISPR

Expression

Mammalian

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

Plasmid

#127353

Purpose

gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and mVenus

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

Plasmid

#127353

Purpose

gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and mVenus

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLZts

Plasmid

#128799

Purpose

Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

Depositor

Timothy Barnett

Publication

Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

Type

Empty backbone

Use

Bacterial mutagenesis

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLZts

Plasmid

#128799

Purpose

Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

Depositor

Timothy Barnett

Publication

Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

Type

Empty backbone

Use

Bacterial mutagenesis

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Publication

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Publication

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA-Cas9-T2A-TdT

Plasmid

#126424

Purpose

Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Promoter

pCMV

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-Cas9-T2A-TdT

Plasmid

#126424

Purpose

Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Promoter

pCMV

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-CD40L-mgSrtA

Plasmid

#125795

Purpose

for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.

Depositor

P. Chen

Publication

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L-mgSrtA

Expression

Mammalian

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD40L-mgSrtA

Plasmid

#125795

Purpose

for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.

Depositor

P. Chen

Publication

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L-mgSrtA

Expression

Mammalian

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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V1-STING

Plasmid

#124263

Purpose

N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Tags

Venus1

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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V1-STING

Plasmid

#124263

Purpose

N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments

Depositor

Eric Schirmer

Insert

TMEM173 (STING1 Human)

Tags

Venus1

Expression

Mammalian

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL1 WT

Plasmid

#123100

Purpose

Expresses 3xFLAG-GABARAPL1 wild-type in mammalian cells.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL1 WT

Plasmid

#123100

Purpose

Expresses 3xFLAG-GABARAPL1 wild-type in mammalian cells.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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