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ISG15 C-term. Domain intein/chitin binding domain (79-156)

Plasmid

#110760

Purpose

Bacterial expression of ISG15 C-term.

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-CAD

Plasmid

#73567

Purpose

Soluble activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

CAD (STIM1 342-448) (STIM1 Human)

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARFRP1-GFP

Plasmid

#67408

Purpose

Mammalian expression of hArfrp with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5A-GFP

Plasmid

#67399

Purpose

Mammalian expression of hArl5 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF8-C-terminus

Plasmid

#64673

Purpose

expresses only C terminus of RNF8, amino acids 402-485

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only C-terminus amino acids 402 to 485

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
mcherry p62 delta UBA silent

Plasmid

#187984

Purpose

mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacking UBA domain (aa389-434) but it has the sil…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(D786N)-FLAG

Plasmid

#164975

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D786N, insertion L aa15 (please see depositor co…

Promoter

EF1a

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pmEos2-N1 GBP (GBP-mEos2)

Plasmid

#162876

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein tagged with mEos2

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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YFP-DAXX-mSIM1/2

Plasmid

#134525

Purpose

Expresses YFP-DAXX fusion protein with a mutations in both its 5' and 3' SIMs

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1/2

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG and SIM (IIVL)…

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ISG15 C-term. Domain intein/chitin binding domain (79-156)

Plasmid

#110760

Purpose

Bacterial expression of ISG15 C-term.

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-CAD

Plasmid

#73567

Purpose

Soluble activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

CAD (STIM1 342-448) (STIM1 Human)

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST47-ARFRP1-GFP

Plasmid

#67408

Purpose

Mammalian expression of hArfrp with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5A-GFP

Plasmid

#67399

Purpose

Mammalian expression of hArl5 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF8-C-terminus

Plasmid

#64673

Purpose

expresses only C terminus of RNF8, amino acids 402-485

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only C-terminus amino acids 402 to 485

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-dCas13-NES-eNAT10

Plasmid

#238299

Purpose

Expresses cytoplasmic version of the programmable RNA acetylation system.

Depositor

Won Do Heo

Article

Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

Insert

dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)

Use

CRISPR

Tags

HIV NES (C terminal of dPspCas13b)

Expression

Mammalian

Mutation

H133A for catalytically inactive mutant, deletion…

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM1 flag-cFLIP

Plasmid

#211529

Purpose

Expresses flag-tagged cFLIP (CFLAR)

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

cFLIP (CFLAR Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Puro-TTL-wt-TTF-1

Plasmid

#67657

Purpose

To create a dox-on inducible expression of human wt-TTF-1 cDNA in cells

Depositor

David Mu

Article

Rice et al J Biol Chem. 2013 Jun 7;288(23):16348-60

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Use

Retroviral

Tags

N/A

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCas13-NES-eNAT10

Plasmid

#238299

Purpose

Expresses cytoplasmic version of the programmable RNA acetylation system.

Depositor

Won Do Heo

Article

Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

Insert

dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)

Use

CRISPR

Tags

HIV NES (C terminal of dPspCas13b)

Expression

Mammalian

Mutation

H133A for catalytically inactive mutant, deletion…

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1 flag-cFLIP

Plasmid

#211529

Purpose

Expresses flag-tagged cFLIP (CFLAR)

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

cFLIP (CFLAR Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Puro-TTL-wt-TTF-1

Plasmid

#67657

Purpose

To create a dox-on inducible expression of human wt-TTF-1 cDNA in cells

Depositor

David Mu

Article

Rice et al J Biol Chem. 2013 Jun 7;288(23):16348-60

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Use

Retroviral

Tags

N/A

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge