We narrowed to 24,931 results for: Spr

1. pBC2103-reci

   Plasmid

   #117172

   Purpose

   U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   reci_arCas9 without NLS
   

   Use

   Lentiviral

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GluA3 (px330:2-guideCas9)

   Plasmid

   #169424

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACE2 g3

   Plasmid

   #153013

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS256

   Plasmid

   #73715

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   pU6::sgRNA
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   SapI insertion site

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJH059_proD_Cas12c_DualRNA-GFP_CAM

   Plasmid

   #183076

   Purpose

   Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c tracrRNA and pre-crRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-dSa-VP64

   Plasmid

   #99680

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64

   Mutation

   dead Cas9

   Available Since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRO16Sh2

   Plasmid

   #180604

   Purpose

   Expression of ShCAST under control of Plac with pRO1600 origin.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLac

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

   Plasmid

   #118157

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-AkCas12b-2AeGFP

   Plasmid

   #121946

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AkCas12b
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5

    Plasmid

    #61257

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. OA-1050E (Ubiq-CasRx)

    Plasmid

    #132416

    Purpose

    Express CasRx under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    CasRx
    

    Expression

    Insect

    Promoter

    Ubiq

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

    Plasmid

    #61256

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK2014-AmCas12b

    Plasmid

    #121951

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Tags

    10xHis tag

    Expression

    Bacterial

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-Puro.3xBsmBI

    Plasmid

    #196709

    Purpose

    Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TU6m-gRNAscaffHygroR

    Plasmid

    #165485

    Purpose

    S. pyogenes Cas9 guide RNA expression in tilapia cells

    Depositor

    Dietmar Kueltz

    Article

    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    Oreochromis mossambicus U6

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK2013-AkCas12b

    Plasmid

    #121950

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AkCas12b
    

    Tags

    10xHis tag and 3xHA tag

    Expression

    Bacterial

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. dSp-VP64-RTA

    Plasmid

    #99675

    Purpose

    Expresses dSp Cas9 fused at C term. To VP64 and RTA activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    VP64-RTA

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP2367

    Plasmid

    #160076

    Purpose

    Z. mobilis CRISPRi, promoter C, empty guide, sfGFP

    Depositor

    Jason Peters

    Article

    Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

    Insert

    empty sgRNA
    

    Expression

    Bacterial

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL11197

    Plasmid

    #191784

    Purpose

    SpCas9 expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas9 +13 introns-T-E9
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Construct 7 - CMVp-Cas9-3xNLS-HSVpA

    Plasmid

    #81247

    Purpose

    Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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