We narrowed to 12,230 results for: nsf

Showing: 8561 - 8580 of 12230 results

Results per page:

Sort results by:

pAAV.CMV.RGS4.C-HA

Plasmid

#154847

Purpose

Expresses murine RGS4 protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Regulator of G protein Signaling 4 (Rgs4 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV2/9-CW3SL-mCherry-DrTrkB

Plasmid

#184002

Purpose

AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkB (NTRK2 Human)

Use

AAV

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pscAAV-Tyr

Plasmid

#135616

Purpose

Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-flex-lck-smV5

Plasmid

#196423

Purpose

Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV and Cre/Lox

Tags

Lck

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKII-soCoChR-GFP

Plasmid

#107711

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

CaMKII

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV_Actb HMEJ donor

Plasmid

#97318

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

Plasmid

#129530

Purpose

Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Inserts

codon-optimized AcrIIC3Nme
sgRNA_Rosa26

Use

AAV

Tags

FLAG/NLS

Promoter

U6 and cytomegalovirus-enhancer chicken β-actin p…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRosa26-TG

Plasmid

#40026

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A

Expression

Mammalian

Mutation

deleted nucleotides 1-274 and insertion of FRT, …

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

Plasmid

#121976

Purpose

transfection studies in eukaryotic cells such as COS-7 cells

Depositor

Christophe Erneux

Article

De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

Insert

Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)

Expression

Mammalian

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTarget TMEM97-P2A-mCardinal

Plasmid

#157747

Purpose

Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-ABE-NT-sgRNA

Plasmid

#112734

Purpose

AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

Depositor

Jin-Soo Kim

Article

Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

Insert

ABE-NT-sgRNA

Use

AAV

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1 U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78603

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only
Str-STIM1-NN_ST-SBP-mCherry

Plasmid

#65263

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37087

Purpose

Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-4mtD3cpv

Plasmid

#181874

Purpose

Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

4mtD3cpv

Use

AAV and Adenoviral

Expression

Mammalian

Mutation

the 4mtD3cpv CDS differs from the recorded GeneBa…

Promoter

CaMKIIa

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_GWRev_Dest

Plasmid

#86954

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

Depositor

Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

Plasmid

#199369

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOstar-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1988

Plasmid

#49119

Purpose

AAV plasmid with CAGGS promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-iCre

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAC84-pCR8-dCas9

Plasmid

#48218

Purpose

dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A)

Use

CRISPR; Gateway donor

Tags

HA Tag

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge