We narrowed to 16,918 results for: Emb;

1. pD-MSP1D1

   Plasmid

   #161607

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   D-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans were replaced b…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-mNINJ1-GFP

   Plasmid

   #208775

   Purpose

   Inducibly expresses mouse NINJ1 tagged with GFP

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   mNINJ1 (Ninj1 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2C-3XtbGFP-PEST

   Plasmid

   #69072

   Purpose

   Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   turboGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNMT

   Plasmid

   #42578

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   N-myristoyl tranferase (NMT1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations T690C and T942C

   Promoter

   T7

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pUD-MSP1D1

   Plasmid

   #161608

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans and tyrosines w…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-MPAct-mRuby3-IRES-Puro

   Plasmid

   #155231

   Purpose

   lentiviral MPAct construct

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Lentiviral expression of MPAct-mRuby sensor
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLck-mVenus-C1

   Plasmid

   #84337

   Purpose

   Expresses a mVenus tagged with a membrane localization sequence

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   Lck (MGCVCSSNPELPVAT)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Ran-T24N-mRFP1-polyA

   Plasmid

   #104561

   Purpose

   Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   T24N

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNCX2-mCherry-VPS4A

   Plasmid

   #115334

   Purpose

   expresses mCherry-VPS4A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutation in position 4050 C to T

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCVhygro-F-G9a

    Plasmid

    #41721

    Purpose

    Retroviral expression of human G9a in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9a (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-Ran-Q69L-mRFP1-polyA

    Plasmid

    #104560

    Purpose

    Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Q69L

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-MPAct-mCit-IRES-Neo

    Plasmid

    #155230

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mCitrine sensor
    

    Use

    Lentiviral

    Tags

    mCitrine

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD2

    Plasmid

    #68161

    Purpose

    Universal domesticator plasmid for level 0 parts

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-RanWT-mRFP1-polyA

    Plasmid

    #59750

    Purpose

    Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran-mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-Nup37-EGFP-polyA

    Plasmid

    #104562

    Purpose

    Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Nup37 (Nup37 Mouse)
    

    Use

    Tags

    EGFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-mNINJ1-GFP

    Plasmid

    #208776

    Purpose

    MSCV vector expressing WT mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-LBR-EGFP-polyA

    Plasmid

    #59748

    Purpose

    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C1-FT-mCitrine-EEVEE-CaaX

    Plasmid

    #155225

    Purpose

    MPAct with increased linker flexiblity

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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