Addgene

We narrowed to 28,680 results for: PLE

Showing: 841 - 860 of 28680 results

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mApple-TfR-RUSH

Plasmid

#166906

Purpose

RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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mApple-GPI-RUSH

Plasmid

#166904

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mApple-GPI

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK1

Plasmid

#138692

Purpose

Expresses a human NUAK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK1 human (NUAK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro

Plasmid

#192001

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiblast-Paxillin-mTurqoise

Plasmid

#74206

Purpose

Lentiviral expression of Paxillin-mTurqoise

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

Paxillin

Use

Lentiviral

Tags

mTurqoise

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-CTSB-puro

Plasmid

#158463

Purpose

Constitutive lentiviral expression of human CTSB.

Depositor

Sho Iketani, Alejandro Chavez

Insert

CTSB (CTSB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX303-ARF6-mNeonGreen

Plasmid

#162027

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-opCas12a-6xNLS

Plasmid

#209022

Purpose

Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

opCas12a-6xNLS-Flag-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

FLAG and MYC

Expression

Mammalian

Mutation

E174R/S542R

Promoter

EF-1a

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-2X-P2A-Puro

Plasmid

#110840

Purpose

Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-2X

Use

Lentiviral

Tags

Expression

Mutation

D10A and 2 tandem NLS sequences in the XTEN linker

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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plenti hSyn PKM1

Plasmid

#220920

Purpose

Neuronal specific expression of PKM1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PKM1 (PKM Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2B

Plasmid

#162001

Purpose

Lentiviral expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2B (PIK3C2B Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-mSstr3-EGFP

Plasmid

#200444

Purpose

Lentiviral expression of mouse Sstr3-EGFP

Depositor

Ken-Ichi Takemaru

Insert

Sstr3 (Sstr3 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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mApple-Alpha-5-Integrin-12

Plasmid

#54864

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

Alpha-5 Integrin (ITGA5 Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgMaob-HepmCherry

Plasmid

#192829

Purpose

Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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plenti-X1-Neo-RPN1-HA

Plasmid

#139868

Purpose

Lentiviral expression of HA-tagged (C terminal) human RPN1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

RPN1 (Ribophorin1) (RPN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2-BSD-mApple

Plasmid

#182308

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

hNGN2 (NEUROG2 Human)
bsd-T2A-mycNLS-mApple

Use

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Mutation

Promoter

EF1a and TRE3G

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti TurboRFP BlastR

Plasmid

#102343

Purpose

Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

TurboRFP

Use

Lentiviral

Tags

N/A

Expression

Mutation

Promoter

CMV immediate early

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-MCM7

Plasmid

#31212

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

MCM7 (MCM7 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only