We narrowed to 14,542 results for: TIM

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-CFP

Plasmid

#55794

Purpose

Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-ECFP (ADRB2 Human, Aequorea victoria)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-SA-CAG-Pa01-BFP

Plasmid

#234142

Purpose

Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Insert

Pa01

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) SDC3

Plasmid

#107501

Purpose

Expresses SDC3, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

SDC3 (codon optimized) (SDC3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPICZc-HsACTG1*-thymosinB-8His

Plasmid

#111147

Purpose

Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

actin gamma 1 (ACTG1 Human)

Use

Pichia pastoris integration vector

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

codon-optimised for expression in Pichia pastoris

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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ERBB2 gRNA (BRDN0001148320)

Plasmid

#76378

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB2 (ERBB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pN1 Lck-GCaMP5G

Plasmid

#34924

Purpose

Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

Depositor

Baljit Khakh

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

Lck-GCaMP5G

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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ERBB2 gRNA (BRDN0001148673)

Plasmid

#76377

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB2 (ERBB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC34A1_STOP

Plasmid

#161266

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC34A1 (SLC34A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-2dV-Camui (T305D/T306D)

Plasmid

#220368

Purpose

T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Rat, Synthetic)

Tags

mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

Expression

Mammalian

Mutation

changed both Threonine 305 and 306 to aspartic ac…

Promoter

CAG

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-PdCO-mScarlet

Plasmid

#198507

Purpose

Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-MFSD2A_STOP

Plasmid

#161059

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD2A (MFSD2A Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC20A2_STOP

Plasmid

#161188

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC20A2 (SLC20A2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAC1-V16-hGDNF

Plasmid

#122035

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

hGDNF (GDNF Human)

Use

AAV

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-UAS-zArchon1-KGC-EGFP-ER2

Plasmid

#108427

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zArchon1-KGC-EGFP-ER2

Use

Zebrafish

Promoter

UAS

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer10b-HA-KRAS G12V CO SR

Plasmid

#164928

Purpose

Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

Depositor

Ji Luo

Article

Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

Insert

KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

HA tag

Mutation

Glycine 12 changed to valine; 9 Valine codons are…

Promoter

Ubc promoter

Available Since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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PRKDC gRNA (BRDN0001149021)

Plasmid

#77864

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-2dV-Camui (T286A)

Plasmid

#220367

Purpose

T286A phosphodead mutant of pCAG-2dV-Camui

Depositor

Ryohei Yasuda

Article

Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

Insert

mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)

Tags

mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

Expression

Mammalian

Mutation

changed Threonine 286 to Alanine

Promoter

CAG

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLCO1B1_STOP

Plasmid

#161354

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1B1 (SLCO1B1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-sACE2v2.4(732)-IgG1

Plasmid

#154106

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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