We narrowed to 66,338 results for: nin

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pPBO.ACT001

Plasmid

#182711

Purpose

aTc inducible dCasRx

Depositor

Joseph Schoeniger

Publication

Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

Insert

dCasRx

Use

CRISPR

Tags

2x-FLAG

Expression

Bacterial

Mutation

Catalytically deactivated R295A, H300A, R849A, H8…

Promoter

pTet

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Active

Plasmid

#173072

Purpose

Active SNAP tag at the centrosome

Depositor

Heidi Hehnly

Publication

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Promoter

CMV IE94

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1

Plasmid

#166825

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Promoter

hPGK

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway Cloning

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKL09-GCaMP6f-mScarlet bb118

Plasmid

#158979

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

Depositor

Joel Kralj

Publication

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-mScarlet fusion

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28-6His-SUMO-Nsp10

Plasmid

#154959

Purpose

Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

Depositor

Justin Kollman

Insert

6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)

Tags

6His-Thrombin-SUMO

Expression

Bacterial

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFSW Syt1 WT IRES GFP

Plasmid

#133816

Purpose

Encodes full-length wild type synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Publication

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Promoter

hSyn (human synapsin I promoter)

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NYESO alpha into TRAC HDRT Source (pTR 223)

Plasmid

#112023

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLX313-TP53-P278A

Plasmid

#118015

Purpose

Constitutive expression of mutant human Tumor Protein p53 (TP53)

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

p.Pro278Ala

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CLTA-GFP HDRT Source (pTR 153)

Plasmid

#112016

Purpose

DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

Depositor

Alexander Marson

Publication

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

CLTA-GFP HDRT (CLTA Human)

Use

CRISPR and Synthetic Biology

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS5

Plasmid

#111760

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Publication

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS5-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEXP(V5-APEX2-NES)

Plasmid

#107596

Purpose

Expresses V5-APEX2-NES in mammalian cells

Depositor

Markus Hafner

Publication

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

APEX2-NES

Use

Frt site to insert into hek293 t-rex genome

Tags

NES and V5

Expression

Mammalian

Promoter

CMV Dox inducible

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hnRNPA2_LC_WT

Plasmid

#98657

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Publication

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pUCmini-iCAP-PHP.B2

Plasmid

#103003

Purpose

non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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SaBE3-Gam

Plasmid

#100810

Purpose

Expresses SaBE3-Gam in mammalian cells

Depositor

David Liu

Publication

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE3-Gam

Expression

Mammalian

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-BRPF3

Plasmid

#65383

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF3 (BRPF3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

Plasmid

#48313

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Sumo

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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