We narrowed to 15,730 results for: NOL

1. pGEX Grap-SH2

   Plasmid

   #46434

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grap (GRAP Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 51-160

   Promoter

   Tac

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-DIO(ChRger1-TS-YFP)

   Plasmid

   #127245

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger1-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-DIO(ChRger3-TS-YFP)

   Plasmid

   #127242

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dLbCpf1-BE0ΔiNLS

   Plasmid

   #107684

   Purpose

   Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0ΔiNLS
   

   Expression

   Mammalian

   Mutation

   D832A/E925A/D1148A

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

   Plasmid

   #160575

   Purpose

   LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtU6-26:LbCas12a_DR
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB3038(XII-1 MarkerFree)

   Plasmid

   #73278

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB3040(XII-4 MarkerFree)

   Plasmid

   #73280

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPK1E-1-gfp

   Plasmid

   #48133

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL2317

   Plasmid

   #36743

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FGFR1-TALEN_Right (FGFR1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TAL2316

    Plasmid

    #36742

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FGFR1-TALEN_Left (FGFR1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pBW1997_pExpr-hU6-CRISPR-DECODER

    Plasmid

    #87549

    Purpose

    Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    gRNAs targeting NGN2, MIAT, ACTC1, TTN
    

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Cten-SH2

    Plasmid

    #46421

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Cten (TNS4 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 444-570

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 GTF2A1

    Plasmid

    #67076

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1 (GTF2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGEX Stat2-SH2

    Plasmid

    #46512

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat2 (STAT2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 566-681

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiGuide-Puro-CTRLg2 (CG510)

    Plasmid

    #139456

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX ShcC-SH2

    Plasmid

    #46487

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcC (SHC3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 485-594

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCellFree_G03 BTF3

    Plasmid

    #67059

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 SMAD2

    Plasmid

    #67095

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-LmoCascade-IL1RNcrRNA

    Plasmid

    #126493

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-LmoCascade-HBE1crRNA

    Plasmid

    #126494

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo HBE1 cr06
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge