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pMSCV-PIG-miR-29b-1/miR-29a

Plasmid

#64231

Purpose

Expresses miR-29b-1 and miR-29a in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-29b-1 and miR-29a (MIR29A, MIR29B1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKAR

Plasmid

#60445

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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hCD12

Plasmid

#225541

Purpose

Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

Depositor

David Christianson

Article

Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

Insert

Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)

Tags

His6-MBP tag with cleavable TEV site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Luciferase-IRES-NrasG12D

Plasmid

#60834

Purpose

Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

NrasG12D (Nras Mouse)

Use

Retroviral

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-dDIO-MCS--4x6T

Plasmid

#196422

Purpose

Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Dre/rox; astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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p300 KAT LIC 1M

Plasmid

#233587

Purpose

Bacterial expression of p300 KAT enzyme

Depositor

Scott Showalter

Article

Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

Insert

p300 KAT (EP300 Human)

Tags

6xHis MBP

Expression

Bacterial

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-AML1B

Plasmid

#12426

Purpose

pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

Depositor

Scott Hiebert

Article

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Insert

AML1B (RUNX1 Human)

Expression

Mammalian

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

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N-Myc-53BP1 WT pLPC-Puro

Plasmid

#19836

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-AML1-ETO

Plasmid

#12428

Depositor

Scott Hiebert

Article

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Insert

AML1-ETO fusion (RUNX1, RUNX1T1 Human)

Expression

Mammalian

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-Arch-GFP

Plasmid

#22222

Purpose

Cre-dependent viral expression of Arch-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Has Service

AAV1 and AAV9

Insert

Arch

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

no

Promoter

CAG

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

Plasmid

#52536

Purpose

Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

Depositor

Scott Sternson

Article

Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

Has Service

AAV1

Insert

hM4D-2a-GFP (CHRM4 Human, Synthetic)

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Promoter

CBA

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32477

Purpose

Cre-dependent expression of PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN beta

Plasmid

#40929

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN beta (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only

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NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pHiUGE GS-gRNA (hSyn-Cre) mTubb3

Plasmid

#200369

Purpose

Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Mouse Tubb3 gRNA and Cre

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha

Plasmid

#40928

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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CAHS2 (pBS0249)

Plasmid

#185139

Purpose

For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32475

Purpose

Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G-5HT3HC

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available Since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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