We narrowed to 16,253 results for: Ott
-
Plasmid#158136PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third alanine residu…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG4
Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG
Plasmid#130684PurposeExpresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD453_F489del, E653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG
Plasmid#130683PurposeExpresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD409_E452del, E653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTIM
Plasmid#81198PurposepTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab8 DN
Plasmid#58451Purposeexpression of drosophila YFP Rab8 dominant negative mutantDepositorInsertRab8 (Rab8 Fly)
UseTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 DN
Plasmid#58445Purposeexpression of drosophila YFP-Rab2 dominant negative mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationS20N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 CA
Plasmid#58443Purposeexpression of drosophila YFP-Rab2 constitutively active mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationQ65L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab14 DN
Plasmid#37715DepositorInsertRab14 (Rab14 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS49N (dominant negative)PromoterGAL4Available sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-NC
Plasmid#30226DepositorInsertrhVitronectin with N and C terminus cleavages (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478, 20-62PromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-CAHS3-mEGFP
Plasmid#205006PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.DepositorInsertRvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mEGFP-NLS
Plasmid#205005PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-mEGFP-NLS
Plasmid#205004PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvACT-mEGFP-NLS
Plasmid#205001PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-C
Plasmid#30224DepositorInsertrhVitronectin with C terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478PromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPV-H1-ccdB-mEF1α-RiH
Plasmid#100598PurposepiggyBac vector for sgRNA cloningDepositorTypeEmpty backboneUsePiggybac vectorTagsExpressionMammalianMutationPromoterHuman H1 PolIII promoter, human FTH1 promoter, mo…Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS1-mEGFP-NLS
Plasmid#205013PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Shim_ProRS_TP_GFP_pK7FWG2
Plasmid#202651PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic ProRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_ThrRS_TP_GFP_pK7FWG2
Plasmid#202652PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic ThrRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_TrpRS_TP_GFP_pK7FWG2
Plasmid#202653PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic TrpRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_AspRS_TP_GFP_pK7FWG2
Plasmid#202647PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic AspRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_GlyRS_TP_GFP_pK7FWG2
Plasmid#202648PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic GlyRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_AsnRS_TP_GFP_pK7FWG2
Plasmid#202649PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic AsnRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_MetRS_TP_GFP_pK7FWG2
Plasmid#202650PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic MetRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP
Plasmid#176015PurposeMammalian expression of EGFP. Parton lab clone DFSDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry
Plasmid#176016PurposeMammalian expression of mCherry. Parton lab clone DFQDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
actc1b-GcAMP5
Plasmid#126563PurposeMuscle specific expression of the calcium reporter GcAMP5 in zebrafish. Parton lab clone APTDepositorInsertGcAMP5
UseTagsExpressionMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+(Zeo)-FLAG-muGFP
Plasmid#166726PurposeExpresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cellsDepositorInsertmuGFP
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM-DCA-muGFP
Plasmid#166727PurposeAAV, encodes monomeric ultrastable GFP (muGFP)DepositorInsertmuGFP
UseAAVTagsExpressionMutationPromoterChicken Beta ActinAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Bin1a
Plasmid#176013PurposeMammalian expression of zebrafish EGFP-Bin1a. Parton lab clone HYADepositorInsertBin1a
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Scon_PheRS_TP_pK7fWG2_IVD_FP611
Plasmid#228372PurposeExpresses N-term transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting; proof-of-concept for Plasmid 228371DepositorInsertN-terminal transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting.
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228374PurposeExpresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_CytC_87.18_SHI04112_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228375PurposeExpresses N-term transit peptide from Sapria himalayana Cytochrome C with demonstrated mito targeting; proof-of-concept for Plasmid 228371DepositorInsertN-terminal transit peptide from S. himalayana Cytochrome C with demonstrated mito targeting.
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_GluRS_87.31.3_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228376PurposeExpresses N-term transit peptide from Sapria himalayana Glutamate aminoacyl-tRNA synthetase evm.model.fragScaff_scaffold_87.31.3; determination of S. himalayana GluRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana GluRS evm.model.fragScaff_scaffold_87.31.3
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228378PurposeExpresses N-term transit peptide from Sapria himalayana Methionine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; determination of S. himalayana MetRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana MetRS evm.model.fragScaff scaffold 440.10
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_ThrRS_3895.3_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228380PurposeExpresses N-term transit peptide from Sapria himalayana Threonine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3895.3; determination of S. himalayana ThrRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana ThrRS evm.model.fragScaff scaffold 3895.3
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228381PurposeExpresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2701-AGER-tdTomato
Plasmid#216470Purposedonor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start siteDepositorInserttdTomato
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP
Plasmid#216476Purposelentiviral dual expression of dsRed and tagBFPDepositorInsertsdsRed
tagBFP
UseLentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-Clover
Plasmid#126572PurposeMultisite gateway vector for 3' tagging with Clover. Parton lab clone DJZDepositorInsertClover
UseTagsExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv
Plasmid#217437PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107-V5
Plasmid#64649Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFkB-TA-LUC-UBC-GFP-W
Plasmid#49343PurposepHAGE NFkB-TA-LUC-UBC-GFP-WDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5/DEST-VPS35
Plasmid#21691DepositorInsertVacuolar Protein Sorting 35 homolog (VPS35 Human)
UseLentiviral; Invitrogen gateway destination vectorTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
mApple-M1 Spastin
Plasmid#134461PurposeM1 Spastin expressionDepositorInsertM1 Spastin (SPAST Human)
UseTagsExpressionMammalianMutationMet to Ala change at amino acid 87PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-p58
Plasmid#166942Purposemammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFPDepositorInsertp58 (Lman1 Rat)
UseTagsGFP and chicken lysozyme signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only