We narrowed to 15,130 results for: pET

1. Human _TFAM_NoMTS_Dimer_pET28a+

   Plasmid

   #60013

   Purpose

   Expresses TFAM No MTS dimerization mutant in bacterial cells

   Depositor

   David Chan

   Article

   Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

   Insert

   Human TFAM No MTS (TFAM Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Missing 1st 42 aas (Mitochondrial targeting seque…

   Promoter

   T7

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET32a-hBTC

   Plasmid

   #199230

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Betacellulin (BTC Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-FAS

   Plasmid

   #49810

   Purpose

   Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   fatty acyl-ACP thioesterase
   hydroxydecanoyl-ACP dehydratase
   b-ketoacyl-ACP synthase III
   3-ketoacyl-ACP reductase
   enoyl-(acyl carrier protein) reductase
   

   Expression

   Bacterial

   Mutation

   Codon-optmized. See supplemental information of …

   Promoter

   T7

   Available Since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_Pro-IL-36γ

   Plasmid

   #214345

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36G (IL36G Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-PSAT

   Plasmid

   #74115

   Purpose

   Expression of PSAT

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   pET28a-PSAT (PSAT1 Human)
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET151-Vps4

   Plasmid

   #87733

   Purpose

   Bacterial expression of full-length S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-3c-rhVTN-C

   Plasmid

   #30224

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with C terminus cleavage (VTN Human)
   

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478

   Available Since

   May 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-MinC

   Plasmid

   #133628

   Purpose

   Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

   Insert

   His-MinC (minC E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-MRPP1

   Plasmid

   #67862

   Purpose

   bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   MRPP1 (TRMT10C Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   mitoch. form (contains aa 40-403)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-T7-Ct-WspR

    Plasmid

    #79166

    Purpose

    Dual expression of Ct biosensor and WspR WT under individual T7 promoters

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Inserts

    WspR (wspR P. areuginosa)
    T7-Spinach2-Ct
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(ICOS)

    Plasmid

    #177903

    Purpose

    Bacterial expression of his10-tagged human ICOS Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    ICOS (aa 162-199 only) (ICOS Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEThT-SAHS2

    Plasmid

    #90026

    Purpose

    To express His-tagged SAHS2 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    SAHS2
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET16b-Mms2

    Plasmid

    #18893

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Yeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
    

    Tags

    HIS10

    Expression

    Bacterial

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pET22b-EC12WTC9AAvi

    Plasmid

    #47925

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Tags

    6xHis, Avi Tag, and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-OmpT

    Plasmid

    #68862

    Purpose

    construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

    Depositor

    Neil Kelleher

    Article

    Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

    Insert

    OmpT (ompT )
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. YPet-Actin-7

    Plasmid

    #56628

    Purpose

    Localization: Actin, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    Actin (ACTB Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pET41a iGluSnFR

    Plasmid

    #119828

    Purpose

    Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETTEV-SENP8

    Plasmid

    #196361

    Purpose

    protein expression of the His-tagged full length protein

    Depositor

    Stefan Knapp

    Insert

    SENP8/NEDP1
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-T5-mad32TAG

    Plasmid

    #188997

    Purpose

    Express madanin-1 with TAG at 32 position

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    madanin-1 with TAG at 32 position
    

    Expression

    Bacterial

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b-T5-mad

    Plasmid

    #188998

    Purpose

    Express wildtype madanin-1

    Depositor

    Han Xiao

    Article

    Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

    Insert

    wildtype madanin-1
    

    Expression

    Bacterial

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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