167,825 results

1. Profiline

   Plasmid

   #89925

   Purpose

   Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

   Depositor

   Guillaume Romet-Lemonne, Antoine Jégou

   Article

   Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

   Insert

   profiline-1 (PFN1 Human)
   

   Tags

   None

   Expression

   Bacterial

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. H6GB1tevP53SS(1-393)

   Plasmid

   #200311

   Purpose

   Bacterial expression of human superstable p53 as His6GB1tev fusion

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (TP53 Human)
   

   Tags

   His6-GB1-tev

   Expression

   Bacterial

   Mutation

   M133L, V203A, N239Y, N268D

   Promoter

   T7

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG PSAM4-5HT3 HC IRES EGFP

   Plasmid

   #119740

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)
   

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F, R420Q, R424D, R428A

   Promoter

   CAG

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-Con/Foff 2.0-mCherry

   Plasmid

   #137133

   Purpose

   Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hs.HRAS G12V

   Plasmid

   #83184

   Purpose

   Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12V mutation

   Depositor

   Dominic Esposito

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12V

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-EFS-HypaSpCas9-P2A-EGFP

   Plasmid

   #106964

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdest EGFP Neo

   Plasmid

   #223052

   Purpose

   EGFP gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIC162

   Plasmid

   #63070

   Purpose

   Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. TK4-NLS-EGFP

   Plasmid

   #239046

   Purpose

   PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

   Depositor

   Marius Wernig

   Inserts

   EGFP
   puroR-T2A-mycNLS-mTagBFP2
   

   Tags

   T2A-mycNLS-mTagBFP2 and c-myc-NLS

   Expression

   Mammalian

   Promoter

   CAG and EF1a

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-C1-p63RhoGEF

    Plasmid

    #67896

    Purpose

    Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-p63RhoGEF
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HA-hIFITM2

    Plasmid

    #58398

    Purpose

    Expressed human IFITM2 with an N-terminal HA tag in mammalian cells

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM2 (IFITM2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. ND4-DdCBE- left side TALE

    Plasmid

    #157844

    Purpose

    expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP12040 - pAAV-3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2040)

    Plasmid

    #223971

    Purpose

    AiE0354m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    NA

    Promoter

    minBG

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TetO-FUW-Lhx2

    Plasmid

    #61537

    Purpose

    Expresses Lhx2 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Lhx2 (Lhx2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGT-Ah7

    Plasmid

    #122574

    Purpose

    MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    catP/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid

    #115160

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-TT loxP 3xP3-DsRed
    

    Use

    Cre/Lox

    Expression

    Insect

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PPP1CA

    Plasmid

    #155843

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PPP1CA (PPP1CA Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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