We narrowed to 11,436 results for: 110

1. pSBL_DK186

   Plasmid

   #156422

   Purpose

   Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein
   

   Use

   Tags

   EGFP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_C2813

   Plasmid

   #91092

   Purpose

   Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4 (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   PvUbi2

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-WRAP53beta

   Plasmid

   #64676

   Purpose

   expresses Wild type WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   WD40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pMC_mNG2(11)_BSDminus_F0

   Plasmid

   #184162

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_B2517

   Plasmid

   #91074

   Purpose

   Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU3

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-rap1-K9R

   Plasmid

   #84527

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   none

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

7. p-715MRPprom-MRPcucuc

   Plasmid

   #87199

   Purpose

   Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   RMRP (RMRP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GAGAG91-95 to CUCUC

   Promoter

   human RMRP

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_C2806

   Plasmid

   #91091

   Purpose

   Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4 (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   FMV 34S

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

   Plasmid

   #127322

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAtCas9-D10A_2

    Plasmid

    #91160

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP8GF-AtTIR1

    Plasmid

    #188427

    Purpose

    Arabidopsis Nuclear Auxin Signaling Receptor TIR1

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

    Plasmid

    #46836

    Purpose

    Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMP-puro-Flag-HA-NR6A1

    Plasmid

    #46833

    Depositor

    Phil Sharp

    Article

    Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

    Insert

    NR6A1 (Nr6a1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag-HA, plus 8-amino acid residue linker

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

14. px330-MRPsg1

    Plasmid

    #87189

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 1

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #1 (RMRP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_A0801

    Plasmid

    #91022

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+) neo with Smad5 CDS

    Plasmid

    #61800

    Purpose

    pcDNA3.1(+) with full length mouse Smad5 CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Smad5 CDS (Smad5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. EYFP-Clathrin-19

    Plasmid

    #56584

    Purpose

    Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pSLiP-G1

    Plasmid

    #188963

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: atcggtcgcattgttttccactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTC365

    Plasmid

    #91214

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD_A1610

    Plasmid

    #91038

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9_D10A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    ZmUbi

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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