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pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

Plasmid

#216166

Purpose

Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)

Use

Lentiviral

Tags

EGFP

Promoter

phU6, ph7SK, phH1, pmU6

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17

Plasmid

#193299

Purpose

tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Klf4-IRES-Sox2-17 (Sox2, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187288

Purpose

Express pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH A740D, E758K, RhoA Q63L

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for flox targeting)

Plasmid

#110815

Purpose

Used with pNTK Gatad2a flox allele targeting construct in mouse cells

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAIP4_1UXZ

Plasmid

#234166

Purpose

Heterologous protein expression of cellulase B in Escherichia coli

Depositor

Christopher Bahl

Insert

1UXZ

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Mouse genome-wide lentiviral CRISPR gRNA library version 2

Pooled Library

#67988

Purpose

Knockout library targeting 18,424 mouse genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Human genome-wide lentiviral CRISPR gRNA library version 1

Pooled Library

#67989

Purpose

Knockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
Green monkey (Chlorocebus sabaeus) sgRNA library

Pooled Library

#178284

Purpose

Knockout library targeting the green monkey (Chlorocebus sabaeus) genome.

Depositor

John Doench, David Root

Article

Wei et al Cell. 2021 Jan 7;184(1):76-91.e13. doi:

Expression

Mammalian

Species

Chlorocebus sabaeus (african green monkey)

Use

CRISPR and Lentiviral

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-OSKM

Plasmid

#136541

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

Plasmid

#193349

Purpose

tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

Plasmid

#193359

Purpose

self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

Plasmid

#224698

Purpose

Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)

Tags

Gly-Gly-6xHis

Expression

Bacterial

Mutation

silent mutation C to T at position 10546 (elimina…

Promoter

T7

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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T7-VEE-CFP-e2a-SOX2-p2a-KLF4

Plasmid

#193358

Purpose

VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHAHA

Plasmid

#16586

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Type

Empty backbone

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
TU#707

Plasmid

#15276

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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TU#711

Plasmid

#15279

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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TU#710

Plasmid

#15278

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
TU#713

Plasmid

#15281

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czyfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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TU#714

Plasmid

#15282

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzcfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
TU#715

Plasmid

#15283

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czcfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge