We narrowed to 24,867 results for: ina
-
Plasmid#135620PurposeExpression of SpyTag-B2AR in insect cellDepositorInsertBeta 2 adrenergic receptor (ADRB2 Human)
TagsHA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tagExpressionInsectMutationDeleted 1-24 aa, 366-413 aaAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ISG15 C-term. Domain intein/chitin binding domain (79-156)
Plasmid#110760PurposeBacterial expression of ISG15 C-term.DepositorAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-Erk D319N
Plasmid#102350PurposeLentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutationDepositorInsertErk2 (Mapk1 Mouse)
UseLentiviralTagsBFPMutationAspartic acid 319 was mutated to asparaginePromoterSFFVAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD mApple
Plasmid#91772PurposeBacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.DepositorInsertmonomeric Apple
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationR97K, E152S, A180S, V207T compared to mApple (FP …PromoteraraBADAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)
Plasmid#74505PurposeOverexpression of CSK SH3-SH2 in mammalian cellsDepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET3a hRegIIIa
Plasmid#64937Purposebacterial expression of processed human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
ExpressionBacterialMutationprocessed form. mutations made in 5' end to …PromoterT7Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
SERA5-bio-His
Plasmid#50817PurposeExpresses enzymatically monobiotinylated full length SERA5DepositorInsertSERA5
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SERA1-bio-His
Plasmid#50812PurposeExpresses enzymatically monobiotinylated full length SERA1DepositorInsertSERA1
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GLURP-bio-His
Plasmid#50802PurposeExpresses enzymatically monobiotinylated full length GLURPDepositorInsertGLURP
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP6-bio
Plasmid#47732PurposeExpresses enzymatically monobiotinylated full-length MSP6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP6
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP5-bio
Plasmid#47718PurposeExpresses enzymatically monobiotinylated full-length MSP5 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP5
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs-EGFP-ERas SSVA-IP
Plasmid#13827DepositorInsertERas SSVA (Eras Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationC224 changed to serineAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE
Plasmid#214265PurposeExpression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.DepositorInsertFlag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
UseAAVTagsEGFPExpressionMammalianMutationN/APromoterSynapsinAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcpAzpaRS_v2
Plasmid#196483PurposeTo express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.DepositorInsertazidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
ExpressionMammalianPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)
Plasmid#211767PurposedCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
Tags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpGK and pEF1aAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR ErbB2-CD3ε-FusionRed
Plasmid#188628PurposeHuman ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensorDepositorInsertERBB2 (ERBB2 Synthetic, Human)
UseLentiviralTagsCD3ε-FusionRedExpressionMammalianPromoterSFFVAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9/CD4-TK2
Plasmid#104397PurposeThe designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.DepositorAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1KD:GFP
Plasmid#105645PurposeKinase-deleted Ikk1(alpha) expression in basal keratinocytesDepositorInserttp63:ikk1KD:GFP (chuk Zebrafish)
TagsGFPExpressionMammalianMutationp63 promoter + ikk1 (kinase domain deleted) + GFPAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-S727A-STAT3-3xFlag
Plasmid#99262PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (S727A)DepositorInsertSignal transducer and activator of transcription 3 - S727A (Stat3 Mouse)
UseAdenoviralTags3x-FlagMutationS727APromoterCMVAvailable SinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Bmpr2-Δ17
Plasmid#49380PurposeInsert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.DepositorInsertBmpr2 (BMPR2 Mouse, Human)
UseLuciferaseMutationchanged nucleotides AC to TG at bp 7347-7348PromoterHuman phosphoglycerate kinase (PGK) promoterAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only