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Plasmid#189766PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarlet3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianMutationencodes the expected internal mutations relative …PromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBITE-EGFRvIII-F271-OKT3
Plasmid#190682PurposeEncodes an EGFRvIII-CD3 BITE protein (F271 clone)DepositorInsertAnti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEFSAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-SLP76-TagBFP
Plasmid#188630PurposeHuman EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensorDepositorInsertEGFR (EGFR Human, Synthetic)
UseLentiviralTagsSLP76-TagBFPExpressionMammalianPromoterSFFVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBsCas12a
Plasmid#174671PurposeExpresses 6xHis-MBP tagged BsCas12aDepositorPublicationInserthuBsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…ExpressionBacterialPromoterT7Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2+ Zf GFP-vcla
Plasmid#185410PurposeGFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMVDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvinculin a (vcla Zebrafish)
UseIn vitro synthesis of mrnaTagseGFPExpressionMammalianAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 tTA SDHB
Plasmid#177977PurposeAll-in-one dox-repressible expression of cDNADepositorInsertSDHB (SDHB Human)
UseLentiviralExpressionMammalianMutationSilent point mutations to SDHB sgRNA targetAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC hGAB.wt V5-His IRES G418
Plasmid#110333PurposeMammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tagsDepositorInsertGLS2 Glutaminase 2 (GLS2 Human)
UseRetroviralTags6xHis and V5ExpressionMammalianPromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNM3689
Plasmid#154111PurposeminiMos single component RMCE landing site vectorDepositorInsertloxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
UseMinimosPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
HC25 pComb3xss anti-Homer1 nanobody
Plasmid#134718PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
GC83 pComb3xss anti-Gephyrin nanobody
Plasmid#134721PurposeBacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Gephyrin (Human) recombinant llama nanobody GC83 (GPHN L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NLS
Plasmid#118938PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
ExpressionPlantAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-dSpCas9
Plasmid#92113PurposeExpression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdead/inactive SpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840APromoterCbhAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SH4-FRB-mRFP
Plasmid#100741PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFyn
TagsFRB and mRFPExpressionMammalianMutationSH4 domain onlyPromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]
Plasmid#84485PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianPromoterEF1α, 1.1 kb long (short version)Available sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p35S::AtSOBIR1-mCherry
Plasmid#86166PurposeSOBIR1-mCherry expressionDepositorAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sema6a.a-Fc-His
Plasmid#72163PurposeExpresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Sema6a.a-AP-His
Plasmid#72037PurposeExpresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
P12-bio
Plasmid#68516PurposeExpresses full-length P. vivax P12 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequenceDepositorInsertP12
Tagsrat CD4 d3+4, enzymatic biotinylation sequenceExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP1-bio
Plasmid#68505PurposeExpresses full-length P. vivax MSP1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequenceDepositorInsertMSP1
Tagsrat CD4 d3+4, enzymatic biotinylation sequenceExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
myc-NLRC5 deltaDD
Plasmid#37511DepositorInsertNLRC5 (NLRC5 Human)
TagsmycExpressionMammalianMutationlacking the N-terminal domain (deletion: aa 1-134)PromoterCMVAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by