167,846 results

1. AAVS1-TRE-SHH

   Plasmid

   #134350

   Purpose

   Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

   Depositor

   Lorenz Studer

   Article

   Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

   Insert

   SHH (SHH Human)
   

   Expression

   Mammalian

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-DIO-tdTomato

   Plasmid

   #72254

   Purpose

   Express tdTomato under EF-1 promoter when Cre is expressed by another vector

   Depositor

   Kazuhiro Oka

   Insert

   tdTomato
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   elongation factor-1

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-SAM-Dest

   Plasmid

   #102563

   Purpose

   Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   N/A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-TODestFLAGHA_RBPMS2

   Plasmid

   #59390

   Purpose

   Destination vector with RBPMS2 for stable cell line generation

   Depositor

   Thomas Tuschl

   Article

   Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

   Insert

   RBPMS2 (RBPMS2 Human)
   

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   Feb. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCdrA-gfp(ASV)C

   Plasmid

   #111615

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Sac-Abeta(M1-42)

   Plasmid

   #71875

   Purpose

   Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-42) (APP Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATG9A-mCherry

   Plasmid

   #197393

   Purpose

   Expresses ATG9A in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

   Insert

   ATG9A (ATG9A Human)
   

   Tags

   mCh

   Expression

   Mammalian

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.(ER).iGlucoSnFR2.HaloTag

   Plasmid

   #244073

   Purpose

   ER expression of green glucose sensor with non-responsive HaloTag

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   iGlucoSnFR2.HaloTag
   

   Use

   AAV

   Tags

   Calreticulin and HaloTag

   Available Since

   Sept. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG.(mem).iGlucoSnFR2

    Plasmid

    #244077

    Purpose

    Plasma membrane targeted expression of green glucose sensor

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2
    

    Use

    AAV

    Tags

    IgG kappa leader

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.(ER).iGlucoSnFR2.H348H.HaloTag

    Plasmid

    #244074

    Purpose

    ER expression of green glucose sensor (high affinity) with non-responsive HaloTag

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.H348H.HaloTag
    

    Use

    AAV

    Tags

    Calreticulin and HaloTag

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG.(cyto).iGlucoSnFR2.H348H.HaloTag

    Plasmid

    #244070

    Purpose

    Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.H348H.HaloTag
    

    Use

    AAV

    Tags

    HaloTag

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.TBG.(cyto).iGlucoSnFR2.mIRFP670nano3

    Plasmid

    #244103

    Purpose

    Cytosolic expression of green glucose sensor with non-responsive mIRFP

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.mIRFP670nano3
    

    Use

    AAV

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_BiLAMP5e3_jGCaMP8m (AAV1)

    Viral Prep

    #213918-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_BiLAMP5e3_jGCaMP8m (#213918). In addition to the viral particles, you will also receive purified pAAV_BiLAMP5e3_jGCaMP8m plasmid DNA. Expression of jGCaMP8m under the control of the Lamp5 interneuron-targeting enhancer E3. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

15. pAAV_BiSSTe10_GCaMP6f (AAV1)

    Viral Prep

    #213818-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV_BiSSTe10_GCaMP6f (#213818). In addition to the viral particles, you will also receive purified pAAV_BiSSTe10_GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the SST interneuron-targeting enhancer E10. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

16. pAAV_BiSSTe4_GCaMP6f (AAV PHP.eB)

    Viral Prep

    #213947-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV_BiSSTe4_GCaMP6f (#213947). In addition to the viral particles, you will also receive purified pAAV_BiSSTe4_GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the SST interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

17. pLP16_Lenti Scramble Control

    Plasmid

    #239417

    Purpose

    Negative control Lentiviral plasmid for SpCas9-based CRISPR KO

    Depositor

    Subhojit Roy

    Article

    Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

    Insert

    Scramble sequence
    

    Use

    Lentiviral

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-H2B-mCardinal (JDW 1418)

    Plasmid

    #242553

    Purpose

    Gateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCardinal
    

    Use

    Gateway subcloning

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUW-TetO-CREB3L2

    Plasmid

    #207207

    Purpose

    doxycycline-inducible expression of Human CREB3L2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-CREB3L2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV mTagBFP2-2A-CHMP2B

    Plasmid

    #232000

    Purpose

    Bicistronic expression of CHMP2B along with an mTagBFP2 transfection marker, connected via 2A sequences.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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