167,910 results

1. Lenti-guide-puro mSptlc2 - 2

   Plasmid

   #198494

   Purpose

   lentiviral stable expression of mSptlc2 gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mSptlc2 (Sptlc2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-guide-puro mPHB2-1

   Plasmid

   #198483

   Purpose

   lentiviral stable expression of mPHB2 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mPhb2 (Phb2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-guide-puro mHgs-2

   Plasmid

   #198482

   Purpose

   lentiviral stable expression of mHgs gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mHgs (Hgs Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-guide-puro mCct8 - 1

   Plasmid

   #198503

   Purpose

   lentiviral stable expression of mCct8 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mCct8 (Cct8 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-HA-HsRoquin2_AE

   Plasmid

   #148614

   Purpose

   Mammalian Expression of HsRoquin2

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   HsRoquin2 (RC3H2 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV sgRNA-Hic1 GFP

   Plasmid

   #106953

   Purpose

   Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   anti-Hic1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30-T7-lacO-His-sfGFP

   Plasmid

   #180754

   Purpose

   pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   G140C- Please see depositor comments

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-SLC29A2_STOP

   Plasmid

   #161305

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC29A2 (SLC29A2 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. AAVS1 intron 1 gRNA as2

   Plasmid

   #132394

   Purpose

   Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIGc-Xph20

    Plasmid

    #133024

    Purpose

    Expresses Xph20 in bacteria

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20
    

    Tags

    His10

    Expression

    Bacterial

    Mutation

    S63K

    Promoter

    T7

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

    Plasmid

    #124175

    Purpose

    piggyBac-based Dox-inducible expression (very weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

    Plasmid

    #124174

    Purpose

    piggyBac-based Dox-inducible expression (weak)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2/1

    Plasmid

    #112862

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap1
    

    Use

    AAV

    Promoter

    Truncated P5

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV.rTH.PI.Cre.SV40 (AAV Retrograde)

    Viral Prep

    #107788-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    rTH

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

16. His6-TEV-CRBN-midi

    Plasmid

    #215330

    Purpose

    CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

    Depositor

    Alessio Ciulli

    Article

    Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

    Insert

    CRBN (CRBN Human)
    

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 41-187 followed by a Gly-Ser…

    Promoter

    lacI

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-TFEB-WT-MYC (#647)

    Plasmid

    #99955

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

    Insert

    TFEB (TFEB Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+HyPer7

    Plasmid

    #136466

    Purpose

    Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV9)

    Viral Prep

    #100854-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pTargetF

    Plasmid

    #62226

    Purpose

    Constitutive expression of sgRNA without donor editing template DNA

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pij23119

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLV-EF1A-CD19.8H.8TM.BB.3z

    Plasmid

    #200671

    Purpose

    Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. SpyCatcher003-mi3

    Plasmid

    #159995

    Purpose

    Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyCatcher003-mi3
    

    Tags

    C-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. LentiCRISPRv2GFP

    Plasmid

    #82416

    Purpose

    3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS (P2A)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral Prep

    #162379-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Promoter

    Syn

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

26. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. nlsSalicS1

    Plasmid

    #232190

    Purpose

    Novel FRET-based biosensor for salicylic acid

    Depositor

    Alexander Jones

    Article

    Tang et al Science. 2025 Oct 9;390(6769):188-194. d

    Insert

    nlsSalicS1
    

    Tags

    His-tag

    Expression

    Plant

    Promoter

    p16

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSBbi-RB

    Plasmid

    #60522

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. AAV.CamKII.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep

    #107790-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV.CamKII.GCaMP6s.WPRE.SV40 (#107790). In addition to the viral particles, you will also receive purified AAV.CamKII.GCaMP6s.WPRE.SV40 plasmid DNA. CamKII-driven GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

30. TRUPATH

    Plasmid Kit

    #1000000163

    Purpose

    A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR

    Depositor

    Bryan Roth

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

31. pTRKH2

    Plasmid

    #71312

    Purpose

    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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32. pAAV-Ef1a-DIO EYFP (AAV5)

    Viral Prep

    #27056-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Tags

    EYFP (Cre-dependent)

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

33. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid

    #127289

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

    Inserts

    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and Ascl1 linked via 2A

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CMV-TO-TEVP

    Plasmid

    #116062

    Purpose

    Expresses TEV protease

    Depositor

    Michael Elowitz

    Article

    Gao et al Science. 2018 Sep 21;361(6408):1252-1258

    Insert

    TEVP
    

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HER2 WT

    Plasmid

    #16257

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT7-hMLH1dn for IVT

    Plasmid

    #178114

    Purpose

    Template for in vitro transcription of human MLH1dn

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized) (MLH1 Human)
    

    Use

    Template for in vitro transcription

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

    Plasmid

    #218868

    Purpose

    Mammalian expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEcCas

    Plasmid

    #73227

    Purpose

    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

    Depositor

    Sheng Yang

    Article

    Li et al Acta Biochim Biophys Sin (Shanghai). 202

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR; Red recombinase

    Expression

    Bacterial

    Promoter

    native promoter

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-Cre

    Plasmid

    #13775

    Purpose

    Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge