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163,297 results

Showing: 1761 - 1800 of 163297 results

  6. Dual-sgRNA_hU6-mU6

    Plasmid
    #154194
    Purpose
    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154194-plasmid-map-sequence-id-308648

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  8. pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV9)

    Viral Prep
    #162381-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Tags
    None
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only

  12. MCS-3_Endofin_LgBiT

    Plasmid
    #182574
    Purpose
    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.
    Depositor
    Bradley McConnell
    Article
    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1
    Insert
    zinc finger FYVE-type (ZFYVE16 Human)
    Use
    Tags
    LgBiT (Large BiT)
    Expression
    Mammalian
    Mutation
    FYVE domain Q739-K806
    Promoter
    Herpes simplex virus (HSV)
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    182574-plasmid-map-sequence-id-450120

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  13. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.
    Depositor
    Brigitte Gasser
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-FLEX-tdTomato (AAV5)

    Viral Prep
    #28306-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pDUAL SRBI (GFP)

    Plasmid
    #86979
    Purpose
    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    SCARB1 (SCARB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV/hPGK
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86979-plasmid-map-sequence-id-173289

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  17. pAAV-hSyn-Cre-P2A-dTomato (AAV Retrograde)

    Viral Prep
    #107738-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    June 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV-Syn-ChrimsonR-tdT (AAV9)

    Viral Prep
    #59171-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pCMV-dR8.2 dvpr

    Plasmid
    #8455
    Purpose
    2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8455-plasmid-map-sequence-id-370240

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  30. pAAV.CAG.GCaMP6f.WPRE.SV40 (AAV9)

    Viral Prep
    #100836-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.CAG.GCaMP6f.WPRE.SV40 (#100836). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Tags
    None
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only

  31. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV9)

    Viral Prep
    #105545-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid
    #207805
    Purpose
    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
    Depositor
    Koen Breyne
    Article
    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do
    Insert
    E-NoMi
    Use
    Lentiviral
    Tags
    3xFlag tag, copGFP, mCherry, and nanoluciferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207805-plasmid-map-sequence-id-419158

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  34. pAAV-hsyn-GRAB_DA2m (AAV9)

    Viral Prep
    #140553-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2m (#140553). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2m plasmid DNA. Synapsin-driven expression of 2nd generation medium affinity dopamine sensor GRAB_rDA2m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  35. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

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  38. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV5)

    Viral Prep
    #26969-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV-hSyn-DIO-EGFP (AAV1)

    Viral Prep
    #50457-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1761 - 1800 of 163297 results