We narrowed to 90,284 results for: Mal;

1. pCMV-haA3A-CBE-VA

   Plasmid

   #220893

   Purpose

   Express haA3A-CBE-VA (with SpCas9) in mammalian cells

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-VA
   

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-haA3A-CBE-VA-NG

   Plasmid

   #220896

   Purpose

   Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-VA-NG
   

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPLINTR GFP v1 backbone

   Plasmid

   #179777

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

   Plasmid

   #113890

   Purpose

   Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Avi-His6

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_eA3A-nCas9

   Plasmid

   #163535

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hKLF4 (Plath)

   Plasmid

   #17967

   Depositor

   Kathrin Plath

   Article

   Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

   Insert

   Kruppel-like factor 4 (gut) (KLF4 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

   Plasmid

   #188991

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   F163L, K249R, G328S, G381V, G429D, D480G

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv1_UQCRC2

   Plasmid

   #177982

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting UQCRC2 (UQCRC2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCT.VV.Orf45

   Plasmid

   #12733

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf45
   

   Expression

   Mammalian

   Available Since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

10. DCT.VV.Orf170

    Plasmid

    #12858

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf170
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

11. MDH1-miR-31-PGK-GFP-Blast

    Plasmid

    #31515

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-31 (Mir31 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid

    #98041

    Purpose

    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    Sa-dCas9-NLS-3xFLAG
    

    Use

    CRISPR

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    D10A + H557A

    Promoter

    CMV

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ3 SHP2 CS

    Plasmid

    #8319

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Expression

    Mammalian

    Mutation

    C459S, mutation of essential catalytic cysteine

    Available Since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_GWRev_Dest

    Plasmid

    #86954

    Purpose

    Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

    Depositor

    Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-hA3F-BE3

    Plasmid

    #113414

    Purpose

    Expresses hA3F-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3F-BE3 (APOBEC3F Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 Ub2-UBB+1

    Plasmid

    #23968

    Depositor

    Nico Dantuma

    Article

    Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

    Insert

    Ubiquitin B +1 transcript (UBB Human)
    

    Expression

    Mammalian

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG HDAC5 S259A/S498A

    Plasmid

    #32216

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser259Ala, Ser498Ala

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCMV6M-Pak1 H83L H86L

    Plasmid

    #12211

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PBD (p21-binding domain) mutant: H83L, H86L (see …

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5 GFP RagB Q99L

    Plasmid

    #112749

    Purpose

    express GFP-RagB (Q99L mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    GFP-RagB (Q99L) (RRAGB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Q99L

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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