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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,030 results for: his

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Showing: 9121 - 9140 of 23030 results

  1. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Article
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123461-plasmid-map-sequence-id-417805

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  12. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52319-plasmid-map-sequence-id-237583

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  19. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Article
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    12X His tag, 3XNLS, FLAG, and MBP
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

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  20. pRBC-sfGFP-150TAG

    Plasmid
    #174076
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,
    Depositor
    Ryan Mehl
    Article
    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15
    Insert
    Superfolder GFP
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    T7
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    174076-plasmid-map-sequence-id-345697

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Showing: 9121 - 9140 of 23030 results