We narrowed to 25,285 results for: crispr
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Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CLYBL-TO-hNGN2-BSD-mApple
Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNGN2 (NEUROG2 Human)
ExpressionMammalianAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-15:ACTB-mEGFP
Plasmid#87425PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACTB Homology Arms with mEGFP-linker (ACTB Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLVgag-3xNES-ABE8e-NG
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE7-P2A-EGFP
TagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianPromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050
Pooled library#1000000114PurposeGenome-wide CRISPR gRNA pooled library for inhibition of human genesDepositorAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRIS-PITChv2-Puro-dTAG (BRD4)
Plasmid#91793PurposePITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPuro-P2A-2xHA-FKBP_F36V
UseCRISPRExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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hCRISPRi-v2, genome-wide, top 5 sgRNAs/gene
Pooled library#83969PurposeVersion 2 of the human CRISPRi genome wide libraryDepositorUseLentiviralAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRIS-PITChv2-dTAG-Puro (BRD4)
Plasmid#91796PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP_F36V-2xHA-P2A-Puro
UseCRISPRExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastPromoterGPDAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianPromoterhuman PGK1Available sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eeBxb1
Plasmid#222339PurposePlasmid for expressing evolved and engineered Bxb1 in the mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCodon-optimized evolved and engineered Bxb1
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsplit EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)
Pooled library#1000000053PurposeCRISPR gRNA knockout pooled library for targeting the mouse genomeDepositorAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE N-terminal
Plasmid#137175PurposeAAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE N-terminal; U6-protospacer
UseAAVMutationCas9 D10APromoterCbhAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMV_AA050
Plasmid#216107PurposegDNA expression for Cas9 with ChenF+E trRNA (guide only)DepositorInsertgDNA expression for Cas9 with ChenF+E trRNA
UseCRISPR and Lentiviral; Assembled vectorAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by