We narrowed to 25,348 results for: CHI;

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pME-QFGal4-SV40pA

Plasmid

#155118

Purpose

Middle entry vector containing QFGal4

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Gateway middle entry vector

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1hWT

Plasmid

#35027

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1 (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
mRNA-CleanCap entry vector

Plasmid

#188249

Purpose

Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS2

Plasmid

#196978

Purpose

Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS2 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto

Plasmid

#167904

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_497

Plasmid

#111824

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 477-497

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLSC-5

Plasmid

#62889

Purpose

Lenti vector for expression of inducible split Cas9

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Inserts

SpCas9(574-1368)
SpCas9(2-573)

Use

CRISPR and Lentiviral

Tags

FFBP12, FRB, NES PTK2, NLS SV40, and P2A

Expression

Mammalian

Mutation

*see comment below

Promoter

EFS

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:EGFP

Plasmid

#118850

Purpose

Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

mitfa: EGFP (mitfa Synthetic, Zebrafish)

Use

Zebrafish expression, tol6

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Red Glifon 300

Plasmid

#163115

Purpose

Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon 300

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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PX458_TEAD1_1

Plasmid

#86356

Purpose

Encodes gRNA for 3' target of human TEAD1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TEAD1 (TEAD1 Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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460 p3E-2A-mCherrypA

Plasmid

#195970

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PX458_TEAD3_1

Plasmid

#86337

Purpose

Encodes gRNA for 3' target of human TEAD3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TEAD3 (TEAD3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAJM.336

Plasmid

#108528

Purpose

LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LacIAM + PTac-YFP

Expression

Bacterial

Mutation

LacI Q18M A47V F161Y

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pX330_SpCas9_USP7 Exon 3 gRNA

Plasmid

#131257

Purpose

pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

humanised S. pyogenes Cas9 nuclease

Expression

Mammalian

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.shNkx2-1

Plasmid

#32400

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

shRNA targetting Nkx2-1 (Nkx2-1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Article

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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