We narrowed to 30,748 results for: PLE;

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pDGE495

Plasmid

#153247

Purpose

M5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-ORP5deltaTMD-FKBP

Plasmid

#220073

Purpose

expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5deltaTMD (OSBPL5 Human)

Expression

Mammalian

Mutation

deltaTMD

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDGE336

Plasmid

#153245

Purpose

M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE341

Plasmid

#153250

Purpose

M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTA-HUH

Plasmid

#221152

Purpose

HisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolytica

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Insert

HisG-URA3-HisG

Expression

Bacterial and Yeast

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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BiP 2: C. elegans BiP (hsp-4)-GFP

Plasmid

#21896

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

BiP (hsp-4 Nematode)

Tags

GFP

Expression

Worm

Mutation

Fusion between the GFP and hsp-4 takes place at A…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pJAT11

Plasmid

#204290

Purpose

For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-DsRed

Use

CRISPR

Promoter

ie1

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP wt

Plasmid

#41382

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huErCas12a

Plasmid

#174685

Purpose

Expresses 6xHis-MBP tagged ErCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huErCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis

Expression

Bacterial

Mutation

Q926R mutation is observed as indicated from the …

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-RanWT-mRFP1-polyA

Plasmid

#59750

Purpose

Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran-mRFP1

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p5T7-LYCipi-ggpps

Plasmid

#122017

Purpose

Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Insert

GGPP synthase

Expression

Bacterial

Mutation

codon optimized, truncated first 98 amino acids, …

Promoter

T7

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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inert_EJC

Plasmid

#216224

Purpose

GFP P2A RFP reporter with inert EJC immediately following stop codon

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_inert_EJC

Expression

Mammalian

Available Since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3

Plasmid

#37372

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot3 (CNOT3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pTP728

Plasmid

#216374

Purpose

xNup358-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup358-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Nup37-EGFP-polyA

Plasmid

#104562

Purpose

Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Nup37 (Nup37 Mouse)

Tags

EGFP tag

Expression

Mammalian

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP1

Plasmid

#68434

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR

Plasmid

#181830

Purpose

Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-MRGBP

Plasmid

#15325

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

Insert

MRG-binding protein (MRGBP Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only