We narrowed to 13,828 results for: CAR

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pGTag-TagRFP-B-actin

Plasmid

#117808

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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fck-Jaws-GFP-ER2

Plasmid

#65013

Purpose

Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-GFP-ER2

Use

Lentiviral

Tags

ER2 and GFP

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pATP416-ptetO7-pphlO6-crtYBI

Plasmid

#165976

Purpose

Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Inserts

Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

C1281A, G1698A and C66A, C1359T

Promoter

Synthetic promoter (pphlO6), Synthetic promoter (…

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRECLV103_PGBD5_B

Plasmid

#168053

Purpose

Lentiviral expression vector for fusion of GFP with human PGBD5, NCBI: NM_001258311.1

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPN458

Plasmid

#137876

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL210

Plasmid

#126451

Purpose

Expresses Cas9-XTEN-TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-synaptoPAC-minWPRE

Plasmid

#153100

Purpose

Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-mRFP

Plasmid

#117781

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G-trunc

Plasmid

#158077

Purpose

Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Expression

Mammalian

Mutation

D614G

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLRC1-NEK10p:NEK10-3XFLAG

Plasmid

#137030

Purpose

Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

Depositor

David Sabatini

Article

Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

Insert

NIMA-like kinase 10 (NEK10 Human)

Use

Lentiviral

Tags

3XFLAG

Promoter

human NEK10 endogenous promoter

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63885

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Promoter

EF1a

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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WT1 KI gRNA2

Plasmid

#92313

Purpose

CRISPR-GFP-gRNA for cutting WT1

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Insert

WT1 (WT1 Human)

Tags

CRISPR GFP

Expression

Mammalian

Promoter

U6

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pRepair-mScI-CTNNB1

Plasmid

#153431

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mScarlet-i

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OS

Plasmid

#136576

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7 (zebrafish) WT

Plasmid

#108916

Purpose

Express WT Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7 (hspb7 Zebrafish)

Tags

Halo-TEV

Expression

Mammalian

Promoter

SP6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ai95(RCL-GCaMP6f) targeting vector

Plasmid

#61579

Purpose

Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

CAG-LSL-GCaMP6-fast

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Entry vector

Tags

HA tag

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only