We narrowed to 2,450 results for: LRE
-
Plasmid#222680Purposeoverexpressing full length TSC22D2 with N-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-FL-C-FLAG
Plasmid#222681Purposeoverexpressing full length TSC22D2 with C-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Nterm-N-FLAG
Plasmid#222682Purposeoverexpressing N-terminus truncation of TSC22D2 with N-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 N-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Nterm-C-FLAG
Plasmid#222683Purposeoverexpressing N-terminus truncation of TSC22D2 with C-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 N-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Cterm-N-FLAG
Plasmid#222684Purposeoverexpressing C-terminus truncation of TSC22D2 with N-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 C-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-FMR1-CGG-BsmBI
Plasmid#222966Purpose0CGG with BsmBI site for inserting CGG repeatsDepositorInsertFMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
UseCRISPRTagsExpressionMutationCGG repeats deleted from 5' UTR, BsmBI site …PromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-Cofilin-S41E
Plasmid#186748PurposeExpresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cellsDepositorInsertCofilin-1 (CFL1 Human)
UseTagsGFPExpressionMammalianMutationChanged serine 41 to glutamic acidPromoterCMVAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-Cofilin-S41A
Plasmid#186749PurposeExpresses S41A phosphodead GFP-tagged human cofilin in mammalian cellsDepositorInsertCofilin-1 (CFL1 Human)
UseTagsGFPExpressionMammalianMutationChanged serine 41 to alaninePromoterCMVAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-Cofilin-S41E
Plasmid#186751PurposeExpresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cellsDepositorInsertCofilin-1 (CFL1 Human)
UseTagsmCherryExpressionMammalianMutationChanged serine 41 to glutamic acidPromoterCMVAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3
Plasmid#206093PurposeCav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS
Plasmid#206098Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMV/B-actinAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgErich3#1/Cre
Plasmid#193211PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 geneDepositorInsertsgErich3#1 (4922501L14Rik Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgErich3#2/Cre
Plasmid#193212PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Erich3 geneDepositorInsertsgErich3#2 (4922501L14Rik Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP
Plasmid#187647PurposeEncodes gRNA to correct the SFTPC I73T mutationDepositorInsertI73T gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)
Plasmid#176144PurposeEGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Gly60 to Ala, and a mutation in the P…PromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)
Plasmid#176143PurposeEGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB-T2A-SIRT6 (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutation in Arg65 to Ala, and a mutation in the P…PromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)
Plasmid#176139PurposeEGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassetteDepositorUseLentiviralTagsEGFP and MYCExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro
Plasmid#176089PurposeEGFP fused the N-terminus of POLB containing the mutations Lys35Ala, Lys68Ala, and Lys72Ala, a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationMutations in Lys35 to Ala, Lys68 to Ala, and Lys7…PromoterCMVAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIGF1_P1 Promoter-FLuc2P
Plasmid#154261PurposeDestabilized firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.DepositorInsertIGF-1 canonical P1 Promoter (IGF1 Human)
UseTagsExpressionMammalianMutationPromoterHuman IGF-1 P1 PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIGF1_P2 Promoter-FLuc2P
Plasmid#154262PurposeDestabilized firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.DepositorInsertIGF-1 P2 Promoter (IGF1 Human)
UseTagsExpressionMammalianMutationPromoterHuman IGF-1 P2 PromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry
Plasmid#159631Purposeexpresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoterDepositorInsertAst (AstA Fly)
UseLentiviralTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S12TAG
Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR65Q
Plasmid#92249PurposeHTT donor DNA with 65Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR81Q
Plasmid#92250PurposeHTT donor DNA with 81Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR30Q
Plasmid#92247PurposeHTT donor DNA with 30Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1
Plasmid#184556PurposeKnockdown of murine KRAS gene by targeting the promoter region via CRISPRi systemDepositorInsertKirsten rat sarcoma virus (Kras Mouse)
UseCRISPR, Lentiviral, and Mouse TargetingTagsFlagExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Lamin-A
Plasmid#206027PurposeExpresses GFP-tagged Lamin A in mammalian cellsDepositorInsertLamin-A (LMNA Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX311-SHOC2-V5
Plasmid#134521PurposeLentiviral plasmid expressing SHOC2 with V5 C-term tagDepositorInsertSHOC2 (SHOC2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationSilent mutations made at wobble base position at …PromoterEF1alphaAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-CLDN18
Plasmid#69466PurposeExpresses CLDN18DepositorInsertCLDN18 (CLDN18 Human)
UseRetroviralTagsHA TAGExpressionMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CDK9
Plasmid#23701DepositorInsertCDK9 (CDK9 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-ARHGAP26
Plasmid#69467PurposeExpresses ARHGAP26DepositorInsertARHGAP26 (ARHGAP26 Human)
UseRetroviralTagsHA TAGExpressionMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR45Q
Plasmid#92248PurposeHTT donor DNA with 45Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.2-RBD
Plasmid#184830PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CDK5
Plasmid#23699DepositorInsertCDK5 (CDK5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-OMICRON.BA.1-RBD
Plasmid#184831PurposeMammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertS-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
UseTags6X His tagExpressionMammalianMutationSARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…PromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertWilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CCRK
Plasmid#23717DepositorInsertCCRK (CDK20 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M4/X184PG-IRES(isoform 2)
Plasmid#99568Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM4/X184PGPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M3/Y92C-IRES(isoform 2)
Plasmid#99567Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM3/Y92CPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M2/X184W-IRES(isoform 2)
Plasmid#99566Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM2/X184WPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M1/R141L-IRES(isoform 2)
Plasmid#99565Purposemammalian expression of mutant TMEM230 (isoform 2) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM1/R141LPromoterCMVAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-SNX2-PRDM6
Plasmid#69470PurposeExpresses SNX2-PRDM6 fusion geneDepositorUseRetroviralTagsHA TAGExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CDKL1
Plasmid#23389DepositorInsertCDKL1 (CDKL1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag CDK5
Plasmid#20449DepositorInsertCDK5 (CDK5 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CDK10
Plasmid#23652DepositorInsertCDK10 (CDK10 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag CDK9
Plasmid#20453DepositorInsertCDK9 (CDK9 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M2/X184W-IRES(isoform 1)
Plasmid#99560Purposemammalian expression of mutant TMEM230 (isoform 1) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM2/X184WPromoterCMVAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM230-M1/R141L-IRES(isoform 1)
Plasmid#99559Purposemammalian expression of mutant TMEM230 (isoform 1) and ZsGreenDepositorInsertTMEM230 (TMEM230 Human)
UseTagsExpressionMammalianMutationM1/R141LPromoterCMVAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only