We narrowed to 14,179 results for: CRISPR-Cas9
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Plasmid#138477PurposeExpresses FRB fused SpCas9 for NanoMEDIC packaging.DepositorInsertSpCas9, human codon optimized
UseCRISPRTags3x HA tag and FRBExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …Available SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pScRad52-SpCas9 (pXK-1646)
Plasmid#208823PurposeCRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.DepositorTypeEmpty backboneUseCRISPR; Cas9TagsRad52ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-EGFP_cU6:6-sgRNA
Plasmid#190597PurposeThe plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.DepositorInsertsCas9
sgRNA
UseCRISPRTagsEGFP - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-2A-GFP-noITR
Plasmid#118415PurposeCas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expressionDepositorInsertCas9
UseCRISPRExpressionMammalianAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-mCherry_cU6:6-sgRNA
Plasmid#190598PurposeThe plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoterDepositorInsertsCas9
sgRNA
UseCRISPRTagsmCherry - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-986F (AAEL003877-dCas9-VPR)
Plasmid#183993PurposeExpresses dCas9-VPR under the Ae. aegypti polyubiquitin promoterDepositorInsertAAEL003877-dCAS9-VPR
UseCRISPRTagsdCas9-VPRExpressionInsectPromoterAAEL003887Available SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Kan
Plasmid#232092PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Himar-dCas9-mammalian
Plasmid#138414PurposeProtein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tagDepositorInsertHimar-dCas9
Tags3xFLAG, 6xHis, and SV40 NLSExpressionBacterialPromoterT7Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRTS-GBP-dCas9-mRFP
Plasmid#102934PurposeTetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)DepositorInsertdCas9
UseCRISPRTagsGBP (GFP binding protein and mRFPExpressionMammalianAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
FU_tetO_dCas9-VP192-T2A-EGFP
Plasmid#121173PurposeLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.DepositorInsertdCas9-VP192-T2A-EGFP
UseCRISPR and Lentiviral; Inducible by doxycyclineTagsT2A-EGFP-ires-puroExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX380 Treponema denticola Cas9
Plasmid#68317PurposeTreponema denticola Cas9DepositorInsertTdCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable SinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGB-OCS terminator-SunTag-HA-dCas9-NLS
Plasmid#246798PurposeA GreenGate entry vector containing NLS-dCas9-HA-SunTag fused gene followed by a OCS terminatorDepositorInsertNLS-dCas9-HA-SunTag-OCS terminator
UseGreengate cloning entry vectorAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGH125_dCas9-Blast
Plasmid#85417Purposelentiviral expression vector for dCas9 with Blasticidin selectable markerDepositorInsertdCas9 and Blasticidin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry
Plasmid#197420PurposeCas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNADepositorInsertCas9
UseCRISPRTagsmCherryExpressionMammalianAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_dCas9-5xGCN4_Hygro
Plasmid#235572PurposeDox-inducible expression of dCas9-GCN4 & genomic integrationDepositorInsertdCas9-5xGCN4
UseCRISPRMutationD10A; H840AAvailable SinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cas9m4-VP64
Plasmid#47319PurposeCas9m4 ActivatorDepositorInsertCas9m4-VP64
UseCRISPRAvailable SinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-NED
Plasmid#109358PurposeMammalian vector for expressing effector domains fused to dCas9.DepositorTypeEmpty backboneUseCRISPRTagsdCas9, SV40 NLS, 3xFLAGExpressionMammalianPromoterCMVAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB1_23_dCAS9(3xFLAG_SV40NLS)
Plasmid#161478PurposeBB1 encoding a FLAG tagged dCAS9 with a SV40 NLSDepositorInsertdCAS9
UseCRISPR and Synthetic BiologyTagsFLAG Tag, SV40 NLSAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only