Addgene

We narrowed to 29,554 results for: des.2

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GST-PC-2-CTS

Plasmid

#86068

Purpose

A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-2 (Pkd2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 1-15

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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2. p167 Dync1i1.D

Plasmid

#26439

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform D (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaTdT-2

Plasmid

#228975

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-2

Plasmid

#228962

Purpose

For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-K22R

Plasmid

#220293

Purpose

mammalian expression of Bcl-2-K22R tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

K22R

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G101V

Plasmid

#220295

Purpose

mammalian expression of Bcl-2-G101V tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

G101V

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-K17R

Plasmid

#220292

Purpose

mammalian expression of Bcl-2-K17R tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

K17R

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-ΔBH1

Plasmid

#220297

Purpose

mammalian expression of Bcl-2-ΔBH1 tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

BH1 deleted

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-F104C

Plasmid

#220296

Purpose

mammalian expression of Bcl-2-F104C tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

F104C

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-ΔBH3

Plasmid

#220299

Purpose

mammalian expression of Bcl-2-ΔBH3 tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

BH3 deleted

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-ΔBH2

Plasmid

#220298

Purpose

mammalian expression of Bcl-2-ΔBH2 tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

BH2 deleted

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-T132L

Plasmid

#220303

Purpose

mammalian expression of Bcl-2-T132L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

T132L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-A131L

Plasmid

#220302

Purpose

mammalian expression of Bcl-2-A131L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

A131L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G128L

Plasmid

#220301

Purpose

mammalian expression of Bcl-2-G128L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

G128L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-M.Fas.2

Plasmid

#196688

Purpose

Rep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

STIPTMK insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgGPAA1 guide 2

Plasmid

#193609

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 2 (GPAA1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgAP2M1 guide 2

Plasmid

#193601

Purpose

AP2M1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgAP2M1 guide 2 (AP2M1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgFADD guide 2

Plasmid

#193590

Purpose

FADD knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgFADD guide 2 (FADD Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 2

Plasmid

#193592

Purpose

RELA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 2 (RELA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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