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We narrowed to 31,111 results for: SON

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Showing: 9381 - 9400 of 31111 results

  2. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139097-plasmid-map-sequence-id-273896

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  4. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid
    #223113
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223113-plasmid-map-sequence-id-447636

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  6. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179436
    Purpose
    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    HS4-TRE-cit-HS4-mch (DH)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179436-plasmid-map-sequence-id-355459

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  7. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid
    #169185
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag (nsp12 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169185-plasmid-map-sequence-id-334217

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  8. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156434
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    All zinc fingers mutated
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156434-plasmid-map-sequence-id-310270

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  10. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

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  11. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid
    #223125
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223125-plasmid-map-sequence-id-447703

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  12. NST1_pOpOn2.1

    Plasmid
    #228065
    Purpose
    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.
    Depositor
    Charles Anderson
    Article
    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10
    Insert
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    Use
    Dexamethasone inducible vector
    Expression
    Plant
    Promoter
    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1
    Available Since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228065-plasmid-map-sequence-id-456794

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  13. pαH-S-GSAS-B.1.1.7

    Plasmid
    #171747
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Insert
    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171747-plasmid-map-sequence-id-337765

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  14. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid
    #114077
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R/S542R/K607R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114077-plasmid-map-sequence-id-220525

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  17. pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

    Plasmid
    #197499
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197499-plasmid-map-sequence-id-386708

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  19. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119188-plasmid-map-sequence-id-230532

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  20. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid
    #119189
    Purpose
    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119189-plasmid-map-sequence-id-230531

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Showing: 9381 - 9400 of 31111 results