We narrowed to 10,808 results for: T7

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M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E/R59E/R61E

Plasmid

#60355

Purpose

R53E R59E R61E triple mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E, R59E and R61E mutations; contains amino aci…

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
hSRAP.TWIN1.a13-87

Plasmid

#49816

Purpose

expression plasmid for SRAP(13-87) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-87 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pMCSG49-AlleyCat7

Plasmid

#42591

Depositor

Ivan Korendovych

Article

Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Tags

His and TEV

Expression

Bacterial

Mutation

changed Phenylalanine 92 to Glutamine acid, Isole…

Promoter

T7

Available Since

March 21, 2013

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a13-215

Plasmid

#49817

Purpose

expression vector for SRAP(13-215) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-215 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pJTB115

Plasmid

#248909

Purpose

Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria

Depositor

Edward Winter

Article

Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-

Inserts

SMK1 Mitogen-activated Protein Kinase (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes)
Ssp2 (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)

Tags

glutathione-S-transferase

Expression

Bacterial

Mutation

NdeI and NcoI sites were removed for cloning purp…

Promoter

T7

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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His-AA195-428-GFP

Plasmid

#247241

Purpose

A bacterial expression plasmid encoding Arl13b (aa195-428) with N-terminal His and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13b (ARL13B Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

delete amino acids 1-194

Promoter

T7 Promoter

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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His-13bCTS-GFP

Plasmid

#247243

Purpose

A bacterial expression plasmid encoding ciliary targeting sequence of mammalian Arl13b (amino acids 347-363) with N-terminal His and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

deleted amino acid 1-346,364-428

Promoter

T7 Promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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His-AA1-245-GFP

Plasmid

#247240

Purpose

A bacterial expression plasmid encoding Arl13b (aa1-245) with N-terminal His and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

deleted amino acids 246-428

Promoter

t7

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL2870

Plasmid

#232515

Purpose

Genetic complementation plasmid of Cys-light LetA(L94C,I383C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

[LetA1(1-223, L94C,C124S)]-[LetA2(224-427,I383C,C…

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2871

Plasmid

#232516

Purpose

Genetic complementation plasmid of Cys-light LetA(Q180C,R380C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

[LetA1(1-223,Q180C,C124S)]-[LetA2(224-427,R380C,C…

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2792

Plasmid

#232507

Purpose

Genetic complementation plasmid of LetA(ΔZnR-N ΔZnR-C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA ΔZnR-N ΔZnR-C

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2864

Plasmid

#232513

Purpose

Genetic complementation plasmid of Cys-light LetA with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

[LetA1(1-223, C124S)]-[LetA2(224-427,C266S, C343D…

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET20b-His6-LONP1

Plasmid

#247937

Purpose

Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form)

Depositor

Gabriel Lander

Article

Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:

Insert

LONP1 (LONP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

encodes aa 115-959

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET20b-His6-LONP1-Walker B mutant (E591A)

Plasmid

#247938

Purpose

Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form) and Walker B mutant (E591A)

Depositor

Gabriel Lander

Article

Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:

Insert

LONP1 (LONP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

encodes aa 115-959, with E591A

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-N pc4789

Plasmid

#229753

Purpose

Bacterial expression plasmid of MBD2-N (N-terminus of MBD2: aa 1-152). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

N-terminus of MBD2: aa 1-152

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-MBDTRD pc4791

Plasmid

#229754

Purpose

Bacterial expression vector of MBD2-MBDTRD domains (aa 153-235) C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2-MBDTRD domains (aa 153-235)

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆C pc4792

Plasmid

#229755

Purpose

Bacterial expression plasmid of MBD2∆C lacking the C-terminus (a 236-414). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the C-terminus (a 236-414)

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2-C pc4793

Plasmid

#229756

Purpose

Bacterial expression plasmid of MBD2-C (C-terminus of MBD2: aa 236-414). C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

C-terminus of MBD2: aa 236-414

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆GR pc5078

Plasmid

#229758

Purpose

Bacterial expression plasmid of MBD2∆GR. The Protein is lacking the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the glycine/arginine rich domain: aa…

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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