We narrowed to 31,694 results for: PLE

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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YSBE 524 : pNEO_mCherry

Plasmid

#92084

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, Neo selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

mCherry

Use

tagging proteins in cryptococcus neoforman

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMSC234

Plasmid

#216357

Purpose

xhNup93-Nb3t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup93-Nb3t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKHR5

Plasmid

#74593

Purpose

an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette

Use

Cre/Lox; Zebrafish expression

Promoter

alpha-crystallin

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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PB_TRE_AP4-PDGFR_EF1a_eBFP2

Plasmid

#186306

Purpose

Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP4-PDGFR
eBFP2

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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BiP 2: C. elegans BiP (hsp-4)-GFP

Plasmid

#21896

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

BiP (hsp-4 Nematode)

Tags

GFP

Expression

Worm

Mutation

Fusion between the GFP and hsp-4 takes place at A…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot6L

Plasmid

#37369

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot6L (CNOT6L Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-emerin-EGFP-polyA

Plasmid

#59747

Purpose

Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCk1

Plasmid

#136695

Purpose

Acceptor plasmid for odd level position 1, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-itis pSel

Plasmid

#195344

Purpose

Selection plasmid expressing non-fluorescent EGFP A111V

Depositor

Alexis Komor

Article

Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

Insert

EGFP

Use

CRISPR

Mutation

A111V

Promoter

J23119

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRoquin2_AE

Plasmid

#148614

Purpose

Mammalian Expression of HsRoquin2

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin2 (RC3H2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_CBF-tdTomato_L3-L2

Plasmid

#113708

Purpose

Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

CBF (notch dependent promoter)

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MRGBP

Plasmid

#15325

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

Insert

MRG-binding protein (MRGBP Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV10-hTti1

Plasmid

#30213

Depositor

Noboru Mizushima

Article

Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

Insert

Tel2 interacting protein 1 (TTI1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
Stx3

Plasmid

#186685

Purpose

Expresses Syntaxin 3 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Syntaxin 3

Tags

6 his tag

Expression

Bacterial

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCiVSP(C363S)-SD64TF

Plasmid

#80334

Purpose

Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

Depositor

Yasushi Okamura

Article

Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

Insert

Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)

Use

Xenopus oocyte expression

Mutation

Changed Cysteine 363 to Serine

Promoter

SP6

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1 HA

Plasmid

#8948

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAc5 HA3-eDHFR-T2A-puro

Plasmid

#86395

Purpose

for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

Depositor

David Bentley

Article

Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

Insert

HA3 eDHFR T2A puro

Use

CRISPR

Tags

HA3 eDHFR T2A puro

Mutation

R12Y, G67S and Y100I mutations in E coli DHFR

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28c-F30-2xdBroccoli-47CAG

Plasmid

#237217

Purpose

Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

Depositor

Mingxu You

Article

Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

Insert

47 CAG trinucleotide repeats RNA

Expression

Bacterial

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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