We narrowed to 21,784 results for: Tec

1. psiCHECK-2-TGFBR2-m3_3'UTR

   Plasmid

   #31883

   Depositor

   Robert Blelloch

   Article

   Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

   Insert

   TGFBR2 mutant 3'UTR (TGFBR2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Third miR-302b, 372 7-mer seed match mutated from…

   Promoter

   SV40

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

2. B*07:05:01

   Plasmid

   #203268

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   B*07:05:01 (HLA-B Human)
   

   Expression

   Mammalian

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Zebrafish-gRNA-0005

   Plasmid

   #42245

   Purpose

   gRNA targeted to zebrafish gene gsk3b

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-gsk3b (gsk3b Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

4. mKate2-P2A-APEX2-2xML1N(7Q)

   Plasmid

   #67666

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1Nmut*2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti GW-mCit-PA

   Plasmid

   #113457

   Purpose

   Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCitrine-ProteinA

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLK1 gRNA (BRDN0001144743)

   Plasmid

   #76458

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PLK1 gRNA (BRDN0001147678)

   Plasmid

   #76460

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. [pSK496] pLJC5 HA-Depdc5(AB)

   Plasmid

   #109346

   Purpose

   Lenti-viral expression of HA-tagged Depdc5 mutant AB

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZIKV_NASBA_32B

   Plasmid

   #75011

   Purpose

   Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

   Depositor

   James Collins, Alexander Green

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_NASBA_32B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZIKV_NASBA_27B

    Plasmid

    #75010

    Purpose

    Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_27B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ADCK4 gRNA (BRDN0001145019)

    Plasmid

    #76560

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. NET97 pmRFP-N2 (641)

    Plasmid

    #61994

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    AADACL1 (NCEH1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PTK6 gRNA (BRDN0001149364)

    Plasmid

    #76490

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK6

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK6 (PTK6 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PIP5K1A gRNA (BRDN0001147757)

    Plasmid

    #77963

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PIP5K1A gRNA (BRDN0001147420)

    Plasmid

    #77964

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PIP5K1B gRNA (BRDN0001147624)

    Plasmid

    #77083

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIP5K1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIP5K1B (PIP5K1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA BRCA2 V2102I

    Plasmid

    #139322

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 V2102I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 V2102I using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA BRCA2 E2772K

    Plasmid

    #139323

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 E2772K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 E2772K using base editing
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-HA-Flag-natT1R2 a22-end_S212N

    Plasmid

    #113951

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, S212N substitution

    Promoter

    CMV

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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