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GGE085

Plasmid

#120738

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_Tef

Use

Cloning/assembly plasmid

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p1148-pCSDest-AcrIIA4-mCherry

Plasmid

#126204

Purpose

Mammalian expression of AcrIIA4 fused to mCherry

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIA4

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-mCherry

Plasmid

#105481

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

mCherry

Use

To ganerate riboprobe

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFP no-pA

Plasmid

#80819

Purpose

CMVmin-promoted GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-GFP no pA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pUB6_EPB49

Plasmid

#84015

Purpose

EPB49 minigene to test splicing event

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

EPB49 (DMTN Human)

Expression

Mammalian

Promoter

UB6

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMyc-C1-Cep123 (isoform 3)

Plasmid

#73328

Purpose

Encodes isoform 3 of Cep123 fused to a myc tag

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pC2HP

Plasmid

#63735

Purpose

prey plasmid for an adapted two-hybrid system for C. albicans

Depositor

Patrick Van Dijck

Article

Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

Type

Empty backbone

Use

C. albicans prey plasmid

Tags

FLAG, NLS, and VP domain

Promoter

Met3

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pC2HB

Plasmid

#63734

Purpose

bait plasmid for an adapted two-hybrid system for C. albicans

Depositor

Patrick Van Dijck

Article

Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

Type

Empty backbone

Use

C. albicans bait plasmid

Tags

HA, LexA, and NLS

Promoter

Met3

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + S249

Plasmid

#58908

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S249 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-PTPN11i2

Plasmid

#59613

Purpose

Expression of shRNA against human PTPN11

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PTPN11 (PTPN11 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pET21a-hAIM2-deltaPYD

Plasmid

#51522

Purpose

Express His6-tagged HIN domain of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

His6 and T7

Expression

Bacterial

Mutation

deletion of pyrin domain

Promoter

lac operator

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mAIM2-PYD-EGFP

Plasmid

#51610

Purpose

Express GFP-tagged PYD of mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of HIN domain

Promoter

MESV

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET21 Catalytic Domain of MALT1 Catalytic Mutant

Plasmid

#48971

Purpose

Expresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566; Catalytic Mutant C464A

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
PM-NM!TA

Plasmid

#48651

Purpose

Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (G244R)

Plasmid

#31155

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glycine 244 changed to Arginine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (D380N)

Plasmid

#31248

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Aspartic acid 380 changed to Asparagine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-Val15AlafsX11

Plasmid

#24732

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway Cloning

Mutation

deleted amino acids 44-62. deletion/frameshift…

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pCINeo-hFX(I154F)-HA

Plasmid

#14977

Depositor

Michael Ristow

Article

Schulz et al J Biol Chem. 2006 Jan 13. 281(2):977-81.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Mammalian

Mutation

changed isoleucine 154 to phenylalanine

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) pBluescript

Plasmid

#8531

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Expression

Bacterial

Mutation

makes protein in vitro transcription/translation

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only