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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,228 results for: multi

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Showing: 9541 - 9560 of 31228 results

  8. pThermeleon

    Plasmid
    #86120
    Purpose
    Bandpass temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Publication
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…
    Promoter
    pR/pL, pR/pL, pLacI, and pTlpA
    Available since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86120-plasmid-map-sequence-id-173201

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  9. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73040
    Purpose
    Express Nluc and copGFP dual reporter in mammalian cells
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    PGK (phosphoglycerate kinase 1)
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73040-plasmid-map-sequence-id-136039

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  12. pcDNA3-Lyn-RAB-EKARev

    Plasmid
    #118477
    Purpose
    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-RAB-EKARev
    Tags
    N-terminal targeting sequence from Lyn-kinase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    118477-plasmid-map-sequence-id-243131

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  13. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86619-plasmid-map-sequence-id-168855

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  15. hCMV-IE1:GrRenilla

    Plasmid
    #118067
    Purpose
    Transcriptional unit for constitutive expression of GrRenilla in mammalian cells
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV:GreenRenilla:bGHF
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    118067-plasmid-map-sequence-id-240499

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  17. pSEVA228-pro4IUPi

    Plasmid
    #122018
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Publication
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pro4
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    122018-plasmid-map-sequence-id-238966

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  18. pPB-C6F/TdTomato

    Plasmid
    #140826
    Purpose
    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)
    Depositor
    Hideyuki Okano
    Publication
    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    Expression
    Mammalian
    Mutation
    L104P- please see depositor comments
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140826-plasmid-map-sequence-id-307041

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  19. pX330A_D10A-1x6

    Plasmid
    #58776
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58776-plasmid-map-sequence-id-91086

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Showing: 9541 - 9560 of 31228 results