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pDONR223_MAPK7_p.D46N

Plasmid

#82869

Purpose

Gateway Donor vector containing MAPK7, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAPK7 (MAPK7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D46N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK7_p.T28I

Plasmid

#82814

Purpose

Gateway Donor vector containing MAPK7, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAPK7 (MAPK7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T28I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MMK

Plasmid

#64121

Purpose

a modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

MYC-2A-KLF4 (MYC, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX3_FOXO1

Plasmid

#205875

Purpose

Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX3_FOXO1 (PAX3, FOXO1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TRE-OKSM

Plasmid

#204498

Purpose

Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Inserts

OCT4 (POU5F1 Human)
KLF4 (KLF4 Human)
SOX2 (SOX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKP332 (Lenti-OSK1)

Plasmid

#21627

Depositor

Tim Townes

Article

Chang et al Stem Cells. 2009 May . 27(5):1042-9.

Insert

hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

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FUW-OSKM

Plasmid

#20328

Purpose

Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2K

Plasmid

#20925

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)

Use

Episomal/ebna

Tags

Expression

Mutation

Promoter

Available since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

Plasmid

#210016

Purpose

episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

CAG

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-ASCL1-LMX1b-NURR1-PuroR

Plasmid

#182298

Purpose

Doxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)

Depositor

Kristen Brennand

Article

Powell et al Mol Psychiatry. 2021 Sep 7. pii: 10.1038

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPK13

Plasmid

#20523

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK13 (MAPK13 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

Plasmid

#210017

Purpose

episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for high-quality integration-free naïve reset; best for rodents and primates (including human).

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Sox2A61V is a point mutant of Sox2 that enhances …

Promoter

CAG

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-SOKM

Plasmid

#20325

Purpose

Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223-AURKB

Plasmid

#23682

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKB (AURKB Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2AV-p2a-KLF4

Plasmid

#193291

Purpose

episomal expressing human SOX2A61V + KLF4

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2AV-p2a-KLF4 (SOX2, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

CAG

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_AURKB_WT_V5

Plasmid

#82963

Purpose

Gateway Donor vector containing AURKB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AURKB (AURKB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2-p2a-KLF4

Plasmid

#193292

Purpose

episomal hSK (expressing human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-p2a-KLF4 (SOX2, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

F2A of original pCXLE-SK vector was replaced with…

Promoter

CAG

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK13

Plasmid

#23739

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK13 (MAPK13 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only