We narrowed to 1,383 results for: piggyBac

1. pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

   Plasmid

   #205551

   Purpose

   Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN24-MCP-I
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG::Gdnf

   Plasmid

   #78209

   Purpose

   piggybac vector for mouse Gdnf expression

   Depositor

   Ivo Lieberam

   Article

   Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

   Insert

   Gdnf (Gdnf Mouse)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. AAEL010097-Cas9

   Plasmid

   #100707

   Purpose

   The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL010097-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Promoter

   AAEL010097 and Opie2

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-MPP8_Flag2-Blast

   Plasmid

   #175038

   Purpose

   Mammalian expression of full length mMPP8 with C-terminal Flag2 tag

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   Mphosph8 (Mphosph8 Mouse)
   

   Use

   Piggybac

   Tags

   Flag2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAC150-KEIMA-RAMP4

   Plasmid

   #201929

   Purpose

   PiggyBac Vector expressing KEIMA-RAMP4

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   RAMP4 (SERP1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC150-FAM134C-GFP

   Plasmid

   #201932

   Purpose

   PiggyBac Vector expressing FAM134C-GFP

   Depositor

   Wade Harper

   Article

   Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

   Insert

   FAM134C (RETREG3 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSH231-EF1-GFP-HYGRO

   Plasmid

   #115144

   Purpose

   Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   GFP
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSH231-EF1-RFP-HYGRO

   Plasmid

   #115145

   Purpose

   Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

   Depositor

   Raymond Monnat, Michael Phelps

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   RFP
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

    Plasmid

    #129537

    Purpose

    PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    tetR-3xFlag-HA-2A-Puro
    

    Use

    Synthetic Biology

    Promoter

    hPGK promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKH151_PB_flag-LC3B_wt_IRES-BFP

    Plasmid

    #214976

    Purpose

    Flag tagged LC3B

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-LC3B (MAP1LC3B Human)
    

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Expression

    Insect

    Promoter

    Drosophila melanogaster vasa

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

    Plasmid

    #214977

    Purpose

    Flag tagged LDS mutant LC3B

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-LC3B_mut (MAP1LC3B Human)
    

    Expression

    Mammalian

    Mutation

    LDS mutant

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. BII-Sh-FUCCI-Luc

    Plasmid

    #133364

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Tags

    HA-tagged Clover-Geminin

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAEL005635-Cas9

    Plasmid

    #100608

    Purpose

    The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL005635-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL005635 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC150-KEIMA-REEP5

    Plasmid

    #201928

    Purpose

    PiggyBac Vector expressing KEIMA-REEP5

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    REEP5 (REEP5 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NLS-AgDD

    Plasmid

    #80625

    Purpose

    create nuclear protein aggregates in mammalian cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NLS-NES-L106P-sfGFP (FKBP1A Human)
    

    Tags

    MPPKKKRKVLALKLAGLDI

    Expression

    Mammalian

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC150-TEX264-GFP

    Plasmid

    #201931

    Purpose

    PiggyBac Vector expressing TEX264-GFP

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    TEX264 (TEX264 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid

    #191154

    Purpose

    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    EGFP-t70587
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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