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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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ShadowG-SUMO1

Plasmid

#175239

Purpose

Bacterial expression and purification, conjugatable SUMO for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b SARS-CoV PLpro

Plasmid

#61693

Purpose

inducible expression of SARS PLpro protease

Depositor

Michael Johnson

Article

Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

Insert

SARS-CoV PLpro

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pET28(a)-p2c-Cas9

Plasmid

#242759

Purpose

Expresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coli

Depositor

Jason Rasgon

Article

Chaverra-Rodriguez et al Nat Commun. 2018 Aug 1;9(1):3008. doi: 1

Insert

P2C-Cas9

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-ATG13

Plasmid

#171413

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ATG13 (ATG13 Synthetic)

Expression

Mammalian

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-Mito-7

Plasmid

#55102

Purpose

Localization: Mitochondria, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Mito (COX8A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mNeonGreen

Plasmid

#99135

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V

Plasmid

#63586

Purpose

psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

Depositor

David Rawlings, Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Packaging plasmid for integrase deficient lentiviral vectors

Use

Integrase deficient lentiviral packaging vector

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-Hylight

Plasmid

#193447

Purpose

A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Hylight Biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMax_GFP

Plasmid

#177825

Purpose

Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_EBFP2

Plasmid

#167881

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-mScarlet

Plasmid

#211330

Purpose

Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-2

Plasmid

#172768

Purpose

Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-MCS--4x6T-WPRE

Plasmid

#196417

Purpose

Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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