We narrowed to 23,148 results for: REP
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Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580AAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-KDEL_ManII-SBP-mCherry
Plasmid#65259Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
UseLentiviralTagsStreptavidin Binding Protein (SBP) and mCherryMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP
Plasmid#154139PurposeExpresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.DepositorInsertdCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
Tags3x FLAG and tagBFPExpressionMammalianPromoterCMVAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-OT
Plasmid#37820DepositorInsertPromotorless gfp reporter gene
Available SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHPdestVenus
Plasmid#24568DepositorInsert
UseReporter constructTagsVenusAvailable SinceMay 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUMV19
Plasmid#67161Purposeinducible operation of the mevalonate pathway in E. coliDepositorInsertthe mevalonate pathway genes
ExpressionBacterialPromoterlacZAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA 1 GAPDH
Plasmid#83808PurposeExpress Sg-1 sgRNA targeting GAPDHDepositorInsertSgRNA
UseCRISPRAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Ala9
Plasmid#102616PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).DepositorExpressionBacterialMutationwith an alanine substituted phosphorylation domainPromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 HA TGIF
Plasmid#14048DepositorAvailable SinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-inGFPt
Plasmid#60237PurposeHIV-1 transfer vector that encodes gfp-turbo reporter gene interrupted by intronDepositorInsertinGFPt
UseLentiviralAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mScarletI-NLS-blast
Plasmid#136260PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmScarletI-NLSExpressionMammalianAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sgBFP
Plasmid#192929PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2DepositorInsertSpCas9
TagsT2A mCherryExpressionMammalianAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP
Plasmid#86987PurposeFor cloning and expression of sgRNA together with expression of Cas9 and TagRFPDepositorInsertCas9
ExpressionMammalianPromoterCAGAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypeBacterial Expression, Insect Expression, Mammalian ExpressionCloning TypeMultiSite GatewayAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorMutationFragmentAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.His.XPB
Plasmid#39216DepositorAvailable SinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
NM R2E2 GFP
Plasmid#1208DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutation2 additional copies of second oligopeptide repeat…Available SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
nontarget S. pyogenes scaffold CARGO array
Plasmid#191319PurposeContains an array of nontargeting guide RNAsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM4787
Plasmid#120088PurposeCyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).DepositorInsertPlasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
UseOtherAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only