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Showing: 961 - 980 of 33608 results

  1. Sniper-Cas9

    Plasmid
    #138559
    Purpose
    Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    Sniper-Cas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138559-plasmid-map-sequence-id-298901

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  8. PRE-Hsp70BbCas9_1.2

    Plasmid
    #190796
    Purpose
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190796-plasmid-map-sequence-id-376571

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  9. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  10. pEC1.2/EC1.1_spCas9_TagRFP

    Plasmid
    #161934
    Purpose
    CRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    161934-plasmid-map-sequence-id-320739

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  12. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Tags
    Expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  13. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Propionate inducible promoter (prp)
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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  15. dSaCas9-VPR

    Plasmid
    #205251
    Purpose
    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSaCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205251-plasmid-map-sequence-id-408439

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  16. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Expression
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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Showing: 961 - 980 of 33608 results