We narrowed to 16,015 results for: grn

1. pYPQAT02

   Plasmid

   #223374

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT04

   Plasmid

   #223376

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

   Plasmid

   #232936

   Purpose

   Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   T2A-eGFP-SV40pA with Podxl homology arms
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. act5C-mScarlet

   Plasmid

   #230906

   Purpose

   Ubiquitous expression of mScarlet in Drosophila melanogaster

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. UAST-uMCas12a+nls2x

   Plasmid

   #230909

   Purpose

   inducible expression of Cas12a+ with Gal4.

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   Cas12a+
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGRACE

   Plasmid

   #230910

   Purpose

   Tracing of CRISPR activity in whole animals.

   Depositor

   Michael Boutros

   Article

   Port et al bioRxiv 2024.11.26.625385

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGH643

   Plasmid

   #230834

   Purpose

   hu6-BsmBI-MS2_sgRNA-Ef1a-puroR-T2A-mCherry-wPRE

   Depositor

   Gaelen Hess

   Article

   Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p8390 LentiCRISPRv2 Neo sgNT-2

   Plasmid

   #221650

   Purpose

   Expression of non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

   Insert

   non-targeting sgNT-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GEARBOCS-Vamp2-GeneTRAP

   Plasmid

   #218187

   Purpose

   To KO and genetrap mouse Vamp2 simultaneously

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Insert

   sgRNA (Vamp2 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GEARBOCS-Vamp2-Tag-HA

    Plasmid

    #218188

    Purpose

    To tag endogenous mouse VAMP2 with HA

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Vamp2 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-GFP-2A-Puro-gATRIP_B

    Plasmid

    #211538

    Purpose

    sgRNA-B against ATRIP

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA ATRIP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-GFP-2A-Puro-gHUS1_A

    Plasmid

    #211541

    Purpose

    sgRNA-A against HUS1

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA HUS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-TcCas12b-T2A-EGFP

    Plasmid

    #188277

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

    Insert

    TcCas12b
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-SaCas9-U6-sgKcna2

    Plasmid

    #192796

    Purpose

    Encodes SaCas9 and an sgRNA targeting mouse Kcna2

    Depositor

    Marta Soden

    Article

    Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

    Insert

    sgKcna2
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-FLEX-SaCas9-U6-sgKcna6

    Plasmid

    #192797

    Purpose

    Encodes SaCas9 and an sgRNA targeting mouse Kcna6

    Depositor

    Marta Soden

    Article

    Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

    Insert

    sgKcna6
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. E42_gControl_dTet_IRES_mTurquoise2

    Plasmid

    #189799

    Purpose

    Retroviral delivery of control guide RNA

    Depositor

    Ellen Rothenberg

    Article

    Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

    Insert

    Luciferase gRNA
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Immune checkpoint (library 2) Backbone

    Plasmid

    #192363

    Purpose

    Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T cell activation (library 1) Backbone

    Plasmid

    #192364

    Purpose

    Plasmid vector for in vitro Jurkat activation screening (for immune library)

    Depositor

    Lijia Ma

    Article

    Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiguide-puro_hNKD2sg#1

    Plasmid

    #174323

    Purpose

    gRNA targeting hNKD2 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human NKD2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEDJ-87

    Plasmid

    #163085

    Purpose

    MarkerFree plasmid for integration of pADH1-MCP-VPR into site XI-5

    Depositor

    Michael Jensen

    Article

    Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

    Insert

    VPR
    

    Use

    Tags

    MCP

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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