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pLenti PA-mCit-GW

Plasmid

#113456

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

hUbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G13-G12

Plasmid

#25742

Purpose

Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 and 12 miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pSN65

Plasmid

#100123

Purpose

expresses putative SOX2 DBD with a V5 tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-K660N

Plasmid

#45708

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660N

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 G399S

Plasmid

#16158

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

G399S

Promoter

T7

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
VP-hAR1-660

Plasmid

#89081

Purpose

mammalian expression of human androgen receptor N-terminal fragment 1-660 amino acids linked to VP16 activation domain

Depositor

Elizabeth Wilson

Article

Langley et al J Biol Chem. 1998 Jan 2;273(1):92-101.

Insert

hAR-1-660 (AR Human)

Tags

VP16

Expression

Mammalian

Mutation

aa 1-660 (contains exons A,B,C and part of D)

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pFUSEss-CHIg-mG3_Lys322Arg

Plasmid

#105862

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 heavy chain (Ighg3 Mouse)

Expression

Mammalian

Mutation

Lys322Arg in constant region of IgG1 heavy chain

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

Plasmid

#69894

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Rpb1-linker

Plasmid

#138468

Purpose

Expresses GST-tagged Rpb1 linker in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1460-1585

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-W290C

Plasmid

#45700

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

W290C

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-mu24-ORF24-2xStrep

Plasmid

#138453

Purpose

Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24-ORF24 (ORF24 MHV68/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

mu24 amino acids 1-191 fused to ORF24 amino acids…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_1

Plasmid

#138347

Purpose

Express guide RNA 1 for Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgDnmt1_2

Plasmid

#138348

Purpose

Express guide RNA 2 for mouse Dnmt1

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

mouse Dnmt1 sgRNA (Dnmt1 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-mDIXDC1 WT

Plasmid

#61216

Purpose

Entry clone encoding long isoform of mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

DIXDC1 (Dixdc1 Mouse)

Use

Donr clone (gateway)

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-HisA-HOXC6-1

Plasmid

#110366

Purpose

Mammalian expression of HisA-tagged HOXC6-1

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Tags

HIS

Expression

Mammalian

Mutation

long isoform

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

Plasmid

#108482

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP

Plasmid

#108485

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-Utf1 gRNA

Plasmid

#128842

Purpose

gRNA for targeting mouse Utf1 locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Utf1 gRNA (Utf1 Mouse)

Use

CRISPR

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PA-NL

Plasmid

#113453

Purpose

Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

hUbC

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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