We narrowed to 19,406 results for: Tat
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Plasmid#191392PurposeZinc finger fused to N-terminal part of split mVenusDepositorInsertFlag-NLS-MaSat ZF-18-mVenusN
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ECT2_sgRNA
Plasmid#183873PurposepX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.DepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-STf
Plasmid#181998PurposeCMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell linesDepositorInsertH2B-STf
ExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b-gRNA-NT
Plasmid#184835PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-NT
Plasmid#184568PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPL3-hRPH3A_c.1853Gmut
Plasmid#190479Purposeminigene assayDepositorInsertRPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
ExpressionMammalianMutationc.1853A>GPromoterSV40Available SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-LanCL2-C321A/C367A-C-Ter-His
Plasmid#154188PurposeTo express LanCL2-C321A/C367A in bacterial cellsDepositorInsertLanCL2
TagsHexahistidineExpressionBacterialMutationCys321->Ala321; Cys367->Ala367Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDronpa-N1-Tau-μDpTT
Plasmid#182323PurposeMicrotubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.DepositorInsertTau-μDronpa-TagRFP-T
TagsMAP tauExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLD-CAG-DYSF-CFL
Plasmid#131457PurposeEncodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imagingDepositorExpressionBacterial and MammalianMutationR15C (in DYSF) and Q2579H (in CFL)Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBM0090
Plasmid#172824PurposeLevel 2 module containing mCHERRY-nVENUS HSP21-cVENUSDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
UseSynthetic BiologyAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-GFP-mHP1alpha
Plasmid#181912PurposeBacterial protein expression vector for GFP-tagged HP1alphaDepositorAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Circular 200,100 with interspersed loops (SMAD4)
Plasmid#180191PurposeAAV vector carrying a guide RNA targeting the human SMAD4 mRNADepositorInsertCircular 200,100 guide RNA with interspersed loops
UseAAVExpressionMammalianPromoterHuman U6Available SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 HGV
Plasmid#179230Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
TagsmNeongreenExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 DAT
Plasmid#179229Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
TagsmNeongreenExpressionMammalianMutationG234D, K235APromoterEF-1alphaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
TagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dfnCas12a_crRNA NC
Plasmid#176256PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can beDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseMutationD917A and E1006A mutations to inactivate the endo…Available SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bxb1Donor_G102H_ADRB2_LucReporter
Plasmid#129794Purposeencodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter geneDepositorInsertADRB2 (ADRB2 Human)
UseSynthetic BiologyTags3x FLAGExpressionMammalianMutationG102HPromoterN/AAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-R9-GFP-HiBit
Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM117: VEGFA array presgRNA
Plasmid#166869PurposeU6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.DepositorInsertHuman VEGFA targeting array presgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-GGGS-TDP43 M337V
Plasmid#162612PurposeAllows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assaysDepositorAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only