We narrowed to 66,587 results for: des.1

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRRG43-sFRP1

Plasmid

#99071

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea sFRP1 (secreted frizzled related protein 1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

sFRP1 (secreted frizzled related protein 1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

Plasmid

#46602

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-24 Decoy (Mir24-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

Plasmid

#60418

Purpose

Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

mTiam1-tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

mTiam 64-437 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-B KD/MD

Plasmid

#104450

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein B (VAPB Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

changed K87 to D and M89 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Mutation

Δ754-756 dominant negative mutant

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF1920

Plasmid

#142887

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

Plasmid

#194378

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GLP1R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1

Plasmid

#112858

Purpose

plasmid for expression of rat APOBEC1 in mammalian cells

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-PTK2-C-10

Plasmid

#54240

Purpose

Localization: Focal Adhesion Kinase, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

PTK2 (PTK2 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
HiBiT-GLP1R Fusion Vector

Plasmid

#238559

Purpose

Express HiBiT-GLP1R Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

GLP1R (GLP1R Human)

Tags

HiBiT

Expression

Mammalian

Mutation

Domain 24-463 (native signal peptide deleted)

Promoter

CMV

Available Since

Oct. 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SFFV-ARID4A-Brd 20

Plasmid

#219003

Purpose

Transcription factor ARID4A with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

ARID4A (ARID4A Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (CH.1.1)

Plasmid

#241112

Purpose

Expresses SARS-CoV-2 spike (CH.1.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (CH.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147E,W152R,…

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)

Plasmid

#242559

Purpose

Gateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(Nterm)

Plasmid

#221244

Purpose

Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF N-terminus (1-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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GLG1-2Strep

Plasmid

#210362

Purpose

Expresses GLG1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

GLG1 (GLG1 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT179

Plasmid

#204510

Purpose

Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.

Insert

csr-1a (C. elegans) (csr-1 Nematode)

Tags

6xHis, MBP

Expression

Bacterial

Promoter

T7lac

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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