We narrowed to 59,226 results for: %s

1. pET30b GluBP600n

   Plasmid

   #119835

   Purpose

   Production of recombinant GluBP600n high affinity protein in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   GluBP600n
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. sox10:PDGFRA mutant

   Plasmid

   #98697

   Purpose

   I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish

   Depositor

   Thomas Look

   Article

   Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   exon 8 and 9 deletion

   Promoter

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1kb Zfp521 promoter reporter

   Plasmid

   #104188

   Purpose

   luciferase reporter to assay Zfp521 promoter activation

   Depositor

   Kathryn Hentges

   Article

   Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

   Insert

   Zfp521 promoter - 1 Kb (Zfp521 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   none

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET41a iGlul

   Plasmid

   #119832

   Purpose

   Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGlul
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T92A

   Promoter

   T7

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMyc2ElbLuc,

   Plasmid

   #53244

   Purpose

   reporter plasmid containing 2 myc cDNA binding sites

   Depositor

   Roger Davis

   Article

   Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

   Insert

   pMyc2ElbLuc (MYC Human)
   

   Use

   Tags

   Expression

   Mutation

   To prepare plasmids with one, two, three, and fou…

   Promoter

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pJH3558

   Plasmid

   #179611

   Purpose

   Punc-4::Chrimson::Cherry unc-54 3' UTR C.elegans A-MNs and others expression of Chrimson::Cherry

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   Chrimson
   

   Use

   Tags

   Cherry

   Expression

   Worm

   Mutation

   Promoter

   Punc-4

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-Venus-KRasCT

   Plasmid

   #175834

   Purpose

   Express Venus with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with plasma membrane-targeting sequence
   

   Use

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYC-OspCas12c-noncoding

   Plasmid

   #120878

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-OspCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

   Plasmid

   #172317

   Purpose

   CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZ004 (LseC2c2 locus)

    Plasmid

    #70170

    Purpose

    Expresses LseC2c2 locus with one spacer in array

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    LseC2c2 locus with one spacer in array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pTYF-super-mfPENK-2.2k-Venus-WPRE

    Plasmid

    #156400

    Purpose

    Expresses Venus under PENK gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human T (BRACHYURY) sgRNA

    Plasmid

    #59726

    Purpose

    Plasmid encoding sgRNA to generate T (BRACHYURY) knock-out mutant human cells

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    hT sgRNA (TBXT Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCS2 Flag Smad3 EPSM A208S

    Plasmid

    #27112

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    T179V S204A S208A S213A; A208S

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pCS2 Flag Smad3 EPSM A213S

    Plasmid

    #27113

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    T179V S204A S208A S213A; A213S

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pSN65

    Plasmid

    #100123

    Purpose

    expresses putative SOX2 DBD with a V5 tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBCMV-MS2CP-eDHFR

    Plasmid

    #167309

    Purpose

    Template pDNA to prepare a split CaVT component mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    MS2CP-eDHFR
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTYF-super-mfSERT-1.9k-Venus-WPRE

    Plasmid

    #156394

    Purpose

    Expresses Venus under SERT gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSN16

    Plasmid

    #100103

    Purpose

    expresses a chimeric OCT4-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPK1E-1-gfp

    Plasmid

    #48133

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b-GB

    Plasmid

    #129689

    Purpose

    express E. coli GreB

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    his6/thrombin/GreB (greB E. coli)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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