We narrowed to 23,671 results for: CRISPR

1. PB-PE SpRY

   Plasmid

   #179082

   Purpose

   NOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

   Promoter

   CAG

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ERBB2 gRNA (BRDN0001146057)

   Plasmid

   #76376

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ERBB2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-p300 HAT

   Plasmid

   #179544

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

   Plasmid

   #80769

   Purpose

   Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   IFT88 gRNA#1 (IFT88 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GFAP-NLS-CasRx-NLS-FLAG

   Plasmid

   #154000

   Purpose

   Express CasRx for AAV package

   Depositor

   Hui Yang

   Article

   Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

   Insert

   CasRx
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Promoter

   GFAP

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

   Plasmid

   #71829

   Purpose

   Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   BACH2-sgRNA8 (BACH2 Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; E756A inactiva…

   Promoter

   U6

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAGI1 gRNA (BRDN0001146833)

   Plasmid

   #78067

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAGI1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAGI1 gRNA (BRDN0001487062)

   Plasmid

   #78068

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAGI1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAGI1 gRNA (BRDN0001146934)

   Plasmid

   #78069

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAGI1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAGI1 gRNA (BRDN0001147011)

    Plasmid

    #78070

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAGI1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ERBB2 gRNA (BRDN0001148320)

    Plasmid

    #76378

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERBB2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAPK1 gRNA (BRDN0001149093)

    Plasmid

    #77788

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ERBB2 gRNA (BRDN0001148673)

    Plasmid

    #76377

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERBB2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ABL1 gRNA (BRDN0001147582)

    Plasmid

    #77732

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ABL1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ABL1 (ABL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MAPK1 gRNA (BRDN0001147355)

    Plasmid

    #77787

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PRKDC gRNA (BRDN0001146589)

    Plasmid

    #77861

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKDC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKDC (PRKDC Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HK2 gRNA (BRDN0001162212)

    Plasmid

    #76310

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    HK2 (HK2, HOOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AKT3 gRNA (BRDN0001146868)

    Plasmid

    #76217

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AKT3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AKT3 (AKT3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MAP2K4 gRNA (BRDN0001145028)

    Plasmid

    #76650

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAP2K4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAP2K4 (MAP2K4 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PRKDC gRNA (BRDN0001149021)

    Plasmid

    #77864

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKDC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKDC (PRKDC Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge