Addgene

We narrowed to 230 results for: TIR1

Showing: 81 - 100 of 230 results

Results per page:

Sort results by:

pEN396-pCAGGS-TIR1-V5-2A-HygroR

Plasmid

#195102

Purpose

Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

TIR1

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Use

Tags

Expression

Mammalian

Mutation

Promoter

RPBSA promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN19-3xFLAG-TIR1-NOSt

Plasmid

#108545

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
KJ201: pMVP (R4-R3) osTir1 open

Plasmid

#121743

Purpose

pMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

osTir1 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

9x myc epitope tag

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet-AtTIR1-Myc-MBP-HA

Plasmid

#222709

Purpose

Expression AtTIR1-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtTIR1 (TIR1 Mustard Weed)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Use

Tags

SNAP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PADH1-TIR1-9myc-URA3

Plasmid

#207016

Purpose

Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

TIR1

Use

Tags

9xMyc

Expression

Yeast

Mutation

Promoter

pADH1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
C-SWAT AID*-6MYC osTIR1

Plasmid

#193320

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Use

Tags

6MYC

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C-SWAT AID*-6FLAG osTIR1

Plasmid

#182515

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Use

Tags

6FLAG

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

HTA1

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF2

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-TIR1-tTA/Zeo

Plasmid

#171685

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Zeocin

Use

Tags

Expression

Mammalian

Mutation

Promoter

RPBSA promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN19-TIR1-GUS-NOSt

Plasmid

#108547

Purpose

Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

GUS

Expression

Mutation

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN19-ccvTIR1-GUS-NOSt

Plasmid

#108548

Purpose

Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

GUS

Expression

Mutation

Changed F79 to G

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK444 (EF1-OsTIR1(F74G) Puro)

Plasmid

#214374

Purpose

EF1-OsTIR1(F74G) (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

EF1-OsTIR1(F74G)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1 promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTUB1:OsTIR1-3FLAG, SAG1:CAT

Plasmid

#87258

Purpose

TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

Depositor

David Sibley

Article

Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

Inserts

TIR1
CAT

Use

Toxoplasma expression

Tags

3FLAG

Expression

Mutation

Codon optimized for Toxoplasma gondii expression

Promoter

TgSAG1 361 bp and TgTUB1 2717 bp

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK395 (AAVS1 CMV-OsTIR1(F74A))

Plasmid

#159992

Purpose

Expression of OsTIR1(F74A) in human cells

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)

Plasmid

#140658

Purpose

OsTIR1(WT)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(WT) mAID-EGFP-Nluc TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge