Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 81 - 100 of 118 results

  1. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nunez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

    Enlarge

  2. pDT305

    Plasmid
    #128801
    Purpose
    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Depositor
    Bradley Bernstein
    Article
    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:
    Insert
    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hUBC promoter
    Availability
    Academic Institutions and Nonprofits only
    128801-plasmid-map-sequence-id-255180

    Enlarge

  19. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

    Plasmid
    #186968
    Purpose
    Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.
    Depositor
    Brian Liau
    Article
    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d
    Insert
    pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
    Use
    Lentiviral
    Tags
    rTetR fusion
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha promoter
    Availability
    Academic Institutions and Nonprofits only
    186968-plasmid-map-sequence-id-370475

    Enlarge

  20. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid
    #88879
    Purpose
    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP
    Depositor
    Silvia Monticelli
    Article
    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi
    Insert
    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    Use
    Lentiviral
    Tags
    GFP fusion protein and HA tag
    Expression
    Mammalian
    Mutation
    amino acids 4-460 only; L78P & L287P *see bel…
    Promoter
    SFFV (spleen focus forming virus)
    Availability
    Academic Institutions and Nonprofits only
    88879-plasmid-map-sequence-id-177202

    Enlarge

Showing: 81 - 100 of 118 results