We narrowed to 2,375 results for: pcas9

1. pSpCas9_T2A_mCherry sgAAVS1

   Plasmid

   #192480

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-CBE6b

   Plasmid

   #215820

   Purpose

   Express CBE6b (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-CBE6d

   Plasmid

   #215822

   Purpose

   Express CBE6d (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6d-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-21a_3xNLS_SpCas9_protein_expression

   Plasmid

   #114365

   Purpose

   Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

   Insert

   3xNLS_SpCas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-SpCas9-NG

   Plasmid

   #117919

   Purpose

   Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. B-SpCas9-HF1

   Plasmid

   #126762

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; Q926A; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-nSpCas9

    Plasmid

    #171691

    Purpose

    Expresses nSpCas9 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    EF1a

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. VPR-ps-dSpCas9

    Plasmid

    #102855

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    dSpCas9, pdDronpa1.2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS and VP64-p64-Rta transcription activator

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh and U6

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-eSpCas9_sgAAVS1

    Plasmid

    #199213

    Purpose

    All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    AAVS1-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    human U6

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9 (PX165)

    Plasmid

    #48137

    Purpose

    Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9 TadDE

    Plasmid

    #193837

    Purpose

    Express TadDE (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-DualEditor-Cas9D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpCas9-CBE6c

    Plasmid

    #215821

    Purpose

    Express CBE6c (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SpCas9-mSA*

    Plasmid

    #182952

    Purpose

    Express SpCas9-mSA*

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    SpCas9-mSA*
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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