We narrowed to 16,225 results for: sgRNA

1. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6a:sgRNA#2

   Plasmid

   #64246

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6d:sgRNA#5

   Plasmid

   #64249

   Purpose

   expresses sgRNA under U6d promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA.SFFV.tRFP

   Plasmid

   #169941

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

   Depositor

   Benjamin Ebert

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. esgRNA_NbPDS

   Plasmid

   #231147

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting NbPDS

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   mobile gRNA targeting NbPDS
   

   Expression

   Plant

   Promoter

   PEBV sub-genomic

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459_MCM2_sgRNA

    Plasmid

    #232730

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_CDC45_sgRNA

    Plasmid

    #232732

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. LCV2_AAVS1_sgRNA_2

    Plasmid

    #155088

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. psgRNAc

    Plasmid

    #114006

    Purpose

    express sgRNA, p15A, CRM

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Promoter

    BBa_J23119

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColE1_sgRNA_pos6_AmpR

    Plasmid

    #119149

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PtPuc3_diaCas9_sgRNA

    Plasmid

    #109219

    Purpose

    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

    Depositor

    Per Winge

    Article

    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    LHCF1 terminator, LHCF2 promoter, U6 3' regi…

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCnCas12f1HS_sgRNA_MS13_empty

    Plasmid

    #204999

    Purpose

    CnCas12f1 with MS13 sgRNA site for Human cell genome editing

    Depositor

    Quanjiang Ji

    Article

    Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pColE1_sgRNA_NT_AmpR

    Plasmid

    #119144

    Purpose

    Encodes non-targeting sgRNA

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    non-targeting sgRNA
    

    Use

    Crispr

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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